RBRC03435

Information1

Image
BRC No.RBRC03435
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Arx<tm1Kki>/1Rbrc
Former Common nameArx-1 KO
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Kunio Kitamura, Mitsubishi Kagaku Institute of Life Sciences. E14 ES cells were used to generate the mutant mice.
Strain descriptionArx gene knockout mice. A lacZ-neo cassette was inserted into the exon 2 of Arx gene. Hemizygous (-/Y) mutant males die within 12 hrs after birth. Main line: Arx-6 KO (RBRC03438).
Colony maintenanceHeterozygote (female) x Wild-type (male)[C57BL/6NJcl]
References

Health Report

Examination Date / Room / Rack2013/10/30Room:3-CRack:K
2013/10/21Room:3-CRack:K
2013/10/21Room:3-CRack:K
2013/10/21Room:3-CRack:K
2013/08/26Room:3-CRack:K
2013/07/17Room:3-CRack:K
2013/07/16Room:3-CRack:K
2013/06/17Room:3-CRack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Arxaristaless related homeoboxXArx<tm1Kki>targeted mutation 1, Kunio Kitamura

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)X

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)X

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)Xmouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse ARX genomic DNA, E. coli lacZ, E. coli neo, mouse Pgk promoter, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires)[update, Aug, 14, 2014]
Research applicationFluorescent Proteins/lacZ System
Mouse Models for Human Disease
Specific Term and ConditionsThe BIOLOGICAL RESOURCE shall be used only for academic research purposes by
a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and
negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL
RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL
RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the
source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi
Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical
Corporation of RECIPIENT organization, RECIPIENT name, the specific research
purpose and the date of distribution.
DepositorKunio Kitamura (Mitsubishi Kasei Institute of Life Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Miyabayashi K, Katoh-Fukui Y, Ogawa H, Baba T, Shima Y, Sugiyama N, Kitamura K, Morohashi K.
Aristaless Related Homeobox Gene, Arx, Is Implicated in Mouse Fetal Leydig Cell Differentiation Possibly through Expressing in the Progenitor Cells.
PLoS ONE 8(6) e68050(2013) 23840809