BRC No.RBRC03422
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Apoe<tm1(APOE)Sfu>/129SfuRbrc
Former Common nameApoE4-1 KO
H-2 Haplotype
ES Cell lineE14TG2aIV [129P2/OlaHsd]
Background strain
Strain developmentDeveloped by Shinobu Fujita, Mitsubishi Kagaku Institute of Life Sciences. E14TG2aIV ES cells (129P2/OlaHsd derived) were used. The mice were crossed to C57BL/6.
Strain descriptionHuman ApoE4 knock-in mice line 1 (clone #129 derived). The mouse ApoE sequences including part of exon 2, entire exon 3 and most of exon 4 was replaced by the human APOE4 and a floxed neo casette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]
ReferencesHum Mol Genet. 2000 9(3):353-61. 10655544

Health Report

Examination Date / Room / RackNo Data


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Apoeapolipoprotein E7Apoe<tm1(APOE)Sfu>targeted mutation 1, Shinobu C Fujita

Gene symbolGene nameChr.Allele symbolAllele namePromoter
APOE4apolipoprotein E4 (human)7APOE4

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAhuman APOE4 cDNA, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Apoe genomic DNA
Research applicationCre/loxP system
Hematological Research
Specific Term and ConditionsThe BIOLOGICAL RESOURCE shall be used only for academic research purposes by
a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and
negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL
RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL
RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the
source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi
Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical
Corporation of RECIPIENT organization, RECIPIENT name, the specific research
purpose and the date of distribution.
DepositorShinobu Fujita (Mitsubishi Kasei Institute of Life Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data