Strain Data Sheet

RBRC03390

Information1

Image
BRC No.RBRC03390
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Apoe<tm>/3SfuRbrc
Former Common nameApoE3-3 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Shinobu Fujita, Mitsubishi Kagaku Institute of Life Sciences. 129 derived ES cells were used. The mice were crossed to C57BL/6.
Strain descriptionHuman ApoE3 knock-in mice line 3. The mouse ApoE sequences including part of exon 2, entire exon 3 and most of exon 4 was replaced by the human APOE3 and a floxed neo casette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]
ReferencesHum. Mol. Genet., 9(3):353-361 (2000). 10655544

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Apoeapolipoprotein E7Apoe

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
APOE3apolipoprotein E3 (human)7APOE3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAhuman APOE3 cDNA, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Apoe genomic DNA
Research applicationCardiovascular Research
Cre/loxP system
Hematological Research
Specific Term and ConditionsThe BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution.
DepositorShinobu Fujita (Mitsubishi Kasei Institute of Life Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data