Information1 | |
---|---|
Image | |
BRC No. | RBRC03390 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6;129-Apoe<tm>/3SfuRbrc |
Former Common name | ApoE3-3 KO |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Shinobu Fujita, Mitsubishi Kagaku Institute of Life Sciences. 129 derived ES cells were used. The mice were crossed to C57BL/6. |
Strain description | Human ApoE3 knock-in mice line 3. The mouse ApoE sequences including part of exon 2, entire exon 3 and most of exon 4 was replaced by the human APOE3 and a floxed neo casette. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6NJcl] |
References | Hum. Mol. Genet., 9(3):353-361 (2000). 10655544 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter APOE3apolipoprotein E3 (human)7APOE3 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)7neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Information2 | |
---|---|
Donor DNA | human APOE3 cDNA, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Apoe genomic DNA |
Research application | Cardiovascular Research Cre/loxP system Hematological Research |
Specific Term and Conditions | The BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization. The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation. The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution. |
Depositor | Shinobu Fujita (Mitsubishi Kasei Institute of Life Sciences) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |