Strain Data Sheet

RBRC03337

Information1

Image
BRC No.RBRC03337
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Ttll11<tm1Seto>/SetoRbrc
Former Common nameTTLL11 KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6NJcl
Appearance
Strain developmentDeveloped by Koji Ikegami and Mitsutoshi Setou, Mitsubishi Kagaku Institute of Life Sciences. C57BL/6N derived ES cells (RENKA) were used. The mice were crossed to C57BL/6N.
Strain descriptionTtll11 (TL11) gene knockout mice. Exon 4 was replaced with neo cassette. Initiation codon is in exon 1, therefore exons 1 to 4 were transcribed and translated. Exon 4 codes most of TTL domain (70%). TTL was disrupted by homologous recombination. Expression : Not examined.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]
References

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ttll11tubulin tyrosine ligase-like family, member 112Ttll11<tm1Seto>targeted mutation 1, Mitsutoshi Setou

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2neo; neomycin;

Information2

Donor DNATtll11 genomic DNA (mouse), neo (E. coli)
Research application
Specific Term and ConditionsThe BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution.
DepositorMitsutoshi Setou (Mitsubishi Kasei Institute of Life Sciences)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Wu HY, Rong Y, Bansal PK, Wei P, Guo H, Morgan JI.
TTLL1 and TTLL4 polyglutamylases are required for the neurodegenerative phenotypes in pcd mice.
PLoS Genet 18(4) e1010144(2022) 35404950