Strain Data Sheet

RBRC03328

Information1

Image
BRC No.RBRC03328
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Ttll5<tm1Seto>/SetoRbrc
Former Common nameTL5 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Koji Ikegami and Mitsutoshi Setou, Mitsubishi Kagaku Institute of Life Sciences. 129 derived ES cells were used. The mice were crossed to C57BL/6J.
Strain descriptionTtll5 (TL5) gene knockout mice. Exon 13 was replaced with neo cassette. A region including glutamine acid necessary for activity was deleted (presumably, deficient activity of the enzymes, but not null). Initiation codon is in exon 2. Expression pattern : Not examined.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ttll5tubulin tyrosine ligase-like family, member 512Ttll5<tm1Seto>targeted mutation 1, Mitsutoshi Setou

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)12neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAE. coli Neomycin resistance gene, HSV thymidine kinase promoter, HSV PolyA signal, mouse Ttll5 genomic DNA
Research applicationCell Biology Research
Specific Term and ConditionsThe BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution.
DepositorMitsutoshi Setou (Mitsubishi Kasei Institute of Life Sciences)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Wu HY, Rong Y, Bansal PK, Wei P, Guo H, Morgan JI.
TTLL1 and TTLL4 polyglutamylases are required for the neurodegenerative phenotypes in pcd mice.
PLoS Genet 18(4) e1010144(2022) 35404950