Strain Data Sheet

RBRC03309

Strain Information

Image
BRC No.RBRC03309
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameBIT-212 KO
Former Common nameBIT-212 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Gene structure of mouse BIT/SHPS-1.
Sano S, Ohnishi H, Kubota M
Biochem. J., 344, 667-675 (1999). 10585853

Health Report

Examination Date / Room / Rack2024/04/22Room:3-CRack:I
2024/01/22Room:3-CRack:I
2023/10/23Room:3-CRack:I

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Sirpasignal-regulatory protein alpha2Sirpa
neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal Langerhans cell physiology(MP:0005362)

  • abnormal macrophage physiology(MP:0002451)

  • behavioral despair(MP:0002573)

  • decreased T cell number(MP:0005018)

  • decreased body weight(MP:0001262)
    • more 3 phenotypes
  • hepatic steatosis(MP:0002628)

  • increased megakaryocyte cell number(MP:0008254)

  • thrombocytopenia(MP:0003179)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Sirpa genomic DNA
    Research application
    Specific Term and ConditionsThe BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.
    The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research.
    In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation.
    The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution.
    DepositorSinichiro Sano (Mitsubishi Kasei Institute of Life Sciences)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data