Strain Data Sheet

RBRC03202

Strain Information

Image
BRC No.RBRC03202
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Mgmt<tm1Mse>
Former Common nameMgmt KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Teruhisa Tsuzuki and Mutsuo Sekiguchi, Medical Institute of Bioregulation, Kyushu University in 1996.
Strain description
Colony maintenance
References
Targeted disruption of the DNA repair methyltransferase gene renders mice hypersensitive to alkylating agent.
Tsuzuki T, Sakumi K, Shiraishi A, Kawate H, Igarashi H, Iwakuma T, Tominaga Y, Zhang S, Shimizu S, Ishikawa T et A L
Carcinogenesis., 17, 1215-1220 (1996). 8681434

Enhancement of genomic instability by 17beta-estradiol in minisatellite sequences of X-ray-transformed mouse 10T1/2 cells.
Paquette B
Carcinogenesis., 17, 1221-1225 (1996). 8681435

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
MgmtO-6-methylguanine-DNA methyltransferase7Mgmttargeted mutation 1, Mutsuo Sekiguchi
neoneomycin resistance gene (E. coli)7mouse RNA polymerase II promoter (PolII promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • increased T cell derived lymphoma incidence(MP:0002024)

  • increased physiological sensitivity to xenobiotic(MP:0008873)

  • increased susceptibility to induced morbidity/mortality(MP:0009763)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse mgmt genomic DNA, E. coli Neomycin resistance gene, Mouse RNA polymerase II promoter (PolII promoter), Mouse PolyA signal (Hprt gene)
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Carcinogenesis., 17, 1215-1220 (1996).The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorMutsuo Sekiguchi (Fukuoka Dental College)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data