Strain Data Sheet

RBRC03163

Information1

Image
BRC No.RBRC03163
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6J.129P2-Hmox1<tm1Mym>
Former Common nameHO-1 floxed mouse
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJmsSlc
Appearance
Strain developmentDeveloped by Masayuki Yamamoto, Center for Tsukuba Advanced Research Alliance, Tsukuba University in 2001. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6 (C57BL/6JJcl and C57BL/6JJmsSlc).
Strain descriptionHmox1 gene floxed mice. Exon 3 to 5 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJmsSlc]
ReferencesTohoku J. Exp. Med., 216, 331-339 (2008). 19060448

Health Report

Examination Date / Room / Rack2022/04/25Room:3-CRack:C
2022/03/28Room:3-CRack:C
2022/02/14Room:3-CRack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hmox1heme oxygenase (decycling) 18Hmox1<tm1Mym>targeted mutation 1, Masayuki Yamamoto

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
DsRed2Red Fluorescent Protein (Discosoma sp.)8DsRed2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP8loxP

Information2

Donor DNABacteriophage loxP sites, chimeric intron and splicing acceptor (from pRL-null), Discosoma sp. DsRed2 cDNA, SV40 polyA signal, mouse Heme oxygenase-1 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Tohoku J. Exp. Med., 216, 331-339 (2008).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for TWO years after provided to the RIKEN BRC. Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
DepositorMasayuki Yamamoto (Tohoku University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genetic Background
Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: FP license of TBUSA
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)

BRC mice in Publications

Parada E, Egea J, Buendia I, Negredo P, Cunha AC, Cardoso S, Soares MP, López MG.
The microglial α7-acetylcholine nicotinic receptor is a key element in promoting neuroprotection by inducing heme oxygenase-1 via nuclear factor erythroid-2-related factor 2.
Antioxid Redox Signal 19(11) 1135-48(2013) 23311871

Alves de Souza RW, Gallo D, Lee GR, Katsuyama E, Schaufler A, Weber J, Csizmadia E, Tsokos GC, Koch LG, Britton SL, Wisløff U, Brum PC, Otterbein LE.
Skeletal muscle heme oxygenase-1 activity regulates aerobic capacity.
Cell Rep 35(3) 109018(2021) 33882313

Schallner N, Pandit R, LeBlanc R 3rd, Thomas AJ, Ogilvy CS, Zuckerbraun BS, Gallo D, Otterbein LE, Hanafy KA.
Microglia regulate blood clearance in subarachnoid hemorrhage by heme oxygenase-1.
J Clin Invest 125(7) 2609-25(2015) 26011640

Parada E, Buendia I, Navarro E, Avendaño C, Egea J, López MG.
Microglial HO-1 induction by curcumin provides antioxidant, antineuroinflammatory, and glioprotective effects.
Mol Nutr Food Res 59(9) 1690-700(2015) 26047311

Fernández-Mendívil C, Luengo E, Trigo-Alonso P, García-Magro N, Negredo P, López MG.
Protective role of microglial HO-1 blockade in aging: Implication of iron metabolism.
Redox Biol 38 101789(2020) 33212416