Strain Data Sheet
RBRC03163
Information1 | |
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| Image | |
| BRC No. | RBRC03163 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6J.129P2-Hmox1<tm1Mym> |
| Former Common name | HO-1 floxed mouse |
| H-2 Haplotype | |
| ES Cell line | E14 [129P2/OlaHsd] |
| Background strain | C57BL/6JJmsSlc |
| Appearance | |
| Strain development | Developed by Masayuki Yamamoto, Center for Tsukuba Advanced Research Alliance, Tsukuba University in 2001. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6 (C57BL/6JJcl and C57BL/6JJmsSlc). |
| Strain description | Hmox1 gene floxed mice. Exon 3 to 5 was flanked by loxP sites. |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJmsSlc] |
| References | Tohoku J. Exp. Med., 216, 331-339 (2008). 19060448 |
Health Report | |
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| Examination Date / Room / Rack | 2023/01/23Room:3-CRack:C 2022/10/25Room:3-CRack:C 2022/08/22Room:3-CRack:C 2022/07/25Room:3-CRack:C 2022/04/25Room:3-CRack:C 2022/03/28Room:3-CRack:C 2022/02/14Room:3-CRack:C |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter DsRed2Red Fluorescent Protein (Discosoma sp.)8DsRed2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP8loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP8loxP |
Information2 | |
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| Donor DNA | Bacteriophage loxP sites, chimeric intron and splicing acceptor (from pRL-null), Discosoma sp. DsRed2 cDNA, SV40 polyA signal, mouse Heme oxygenase-1 genomic DNA |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Tohoku J. Exp. Med., 216, 331-339 (2008).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for TWO years after provided to the RIKEN BRC. Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice. |
| Depositor | Masayuki Yamamoto (Tohoku University) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Genetic Background Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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Parada E, Egea J, Buendia I, Negredo P, Cunha AC, Cardoso S, Soares MP, López MG. The microglial α7-acetylcholine nicotinic receptor is a key element in promoting neuroprotection by inducing heme oxygenase-1 via nuclear factor erythroid-2-related factor 2. Antioxid Redox Signal 19(11) 1135-48(2013) 23311871 Alves de Souza RW, Gallo D, Lee GR, Katsuyama E, Schaufler A, Weber J, Csizmadia E, Tsokos GC, Koch LG, Britton SL, Wisløff U, Brum PC, Otterbein LE. Skeletal muscle heme oxygenase-1 activity regulates aerobic capacity. Cell Rep 35(3) 109018(2021) 33882313 Schallner N, Pandit R, LeBlanc R 3rd, Thomas AJ, Ogilvy CS, Zuckerbraun BS, Gallo D, Otterbein LE, Hanafy KA. Microglia regulate blood clearance in subarachnoid hemorrhage by heme oxygenase-1. J Clin Invest 125(7) 2609-25(2015) 26011640 Parada E, Buendia I, Navarro E, Avendaño C, Egea J, López MG. Microglial HO-1 induction by curcumin provides antioxidant, antineuroinflammatory, and glioprotective effects. Mol Nutr Food Res 59(9) 1690-700(2015) 26047311 Fernández-Mendívil C, Luengo E, Trigo-Alonso P, García-Magro N, Negredo P, López MG. Protective role of microglial HO-1 blockade in aging: Implication of iron metabolism. Redox Biol 38 101789(2020) 33212416 |