RBRC03162

Information1

Image
BRC No.RBRC03162
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.B6D2-Tg(Th-EGFP)6-7Koba
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J. Two lines were generated. RBRC02095 (commonly-used), RBRC03162.
Strain descriptionThis transgenic strain expresses GFP under the control of rat tyrosine hydroxylase gene promoter.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesJ. Neurochem., 82, 295-304 (2002). 12124430

Health Report

Examination Date / Room / Rack2010/09/06Room:2-3Rack:C
2010/07/05Room:2-3Rack:C
2010/05/10Room:2-3Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNrat tyrosine hydroxylase (TH) promoter

Information2

Donor DNArat tyrosine hydroxylase (TH) promoter, rabbit beta-globin intron, jelly fish green fluorescent protein (GFP) cDNA, rabbit beta-globin poly A signal, SV40 early gene poly A signal
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Neurochem. 2002 82(2):295-304.
DepositorKazuto Kobayashi (Fukushima Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)

BRC mice in Publications

No Data