RBRC03152

Information1

Image
BRC No.RBRC03152
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain namePcdhg-EN130 dNeo
Former Common nameENdN
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Takeshi Yagi, National Institute for Physiological Sciences/Graduate School of Frontier Biosciences, Osaka University in 2001. Delta neo type (RBRC03152) was generated by crossing with Sycp-cre mice.
Strain descriptionPcdhg gene knockout mice. A polyA of Pcdhg common region was replaced with tau-lacZ gene. lacZ-neo cassette type (RBRC01689), delta neo cassette type (RBRC03152). Homozygous mice show no obvious abnormality.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
ReferencesJ Biol Chem. , 286, 31885-31895 (2011). 21771796

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pcdhgprotocadherin gamma cluster18Pcdhg<tm(EN130dNeo)>targeted mutation

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)18

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)18

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
taubovine tau protein cDNA18tau

Information2

Donor DNAMouse Pcdh-g gene, P1 phage loxP, E. coli LacZ, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), bovine tau
Research application
Specific Term and ConditionsIf the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.
DepositorTakeshi Yagi (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP (Japanese)

BRC mice in Publications

No Data