RBRC03141

Information1

Image
BRC No.RBRC03141
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pcdha<tm(HS5-1)>/TyagRbrc
Former Common namePcdha-HS5-1 conditional KO (+Neo)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University. TT2 ES cells were used.
Strain descriptionPcdha DNase I hypersensitive sites 5, 4, 3, 2, 1(HS5-1) floxed mice (RBRC03141). delta Neo type (RBRC03151), deleted type (RBRC04285).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
ReferencesJ Biol Chem. , 286, 31885-31895 (2011). 21771796

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pcdhaprotocadherin alpha cluster18Pcdha<tm(HS5-1)>targeted mutation

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site18Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)18

Information2

Donor DNAMouse Pcdh-a and Pcdh-b gene, P1 phage loxP, Yeast Frt, E. coli Neomycin resistance gene
Research applicationCre/loxP system
FLP/frt system
Neurobiology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.
DepositorTakeshi Yagi (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data