Strain Data Sheet

RBRC03114

Information1

Image
BRC No.RBRC03114
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(CAG-SCAT3)18Miur
Former Common nameCAG-SCAT3
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Yoshifumi Yamagochi and Masayuki Miura, Graduate School of Pharmaceutical Sciences in 2009.
Strain description
Colony maintenance
ReferencesJ. Cell. Biol., 20, 235-243 (2003). 12527749

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
CFPCyan Fluorescent Protein (Aequorea victoria)UNCFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SCAT3sensor for activated caspases based on FRETUNSCAT3CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
VenusYellow Fluorescent Protein (Aequorea victoria)UNVenusVenus

Information2

Donor DNASCAT3 (Jellyfish ECFP cDNA, caspase-3 cleavage site, Jellyfish Venus cDNA),chicken HS4 insulator, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Biol., 195(6), 1047-1060 (2011).The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasayuki Miura (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (Form V (English)) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
  6. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)

BRC mice in Publications

Konno A, Nishimura A, Nakamura S, Mochizuki A, Yamada A, Kamijo R, Inoue T, Iijima T.
Continuous monitoring of caspase-3 activation induced by propofol in developing mouse brain.
Int J Dev Neurosci 51 42-9(2016) 27126009