Strain Data Sheet

RBRC02975

Information1

Image
BRC No.RBRC02975
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Atg5<tm1Myok>
Former Common nameAtg5flox
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Kenji Nakamura, Mitsubishi Kagaku Institute of Life Sciences and Noboru Mizushima, Tokyo Metropolitan Institute of Medical Science in 2003. The construct was electoroporated into CCE/EK.CCE ES cells derived from 129S/SvEv-Gpi1<c>. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionAtg5 floxed mice. Exon 3 of Atg5 gene was flanked by loxP sites containing neomycin resistant cassette. Autophagy is an intracellular degradation process by an autophagosome, which contains a portion of cytoplasm and subsequently degrades upon fusion with a lysosome. Autophagy is considered to be important for the cellular response to starvation and the normal turnover of cytoplasmic components as well. Atg5, autophagy-related 5 is essential for autophagosome formation. Conditional B6.129S-Atg5<tm1Myok> (RBRC02975) mice can be crossed with various tissue-specific Cre transgenic mice to study the role of autophagy in adult tissues. Null knockout mice are also available: B6;129-Atg5<tm1Nmz> (RBRC02231).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJmsSlc]
ReferencesNature, 441, 885-889 (2006). 16625204

Health Report

Examination Date / Room / Rack2022/03/07Room:4-ARack:B
2021/12/06Room:4-ARack:B
2021/09/08Room:4-ARack:B
2021/06/07Room:4-ARack:B
2021/03/22Room:4-ARack:B
2020/12/14Room:4-ARack:B
2020/10/16Room:4-ARack:B
2020/10/05Room:4-ARack:B
2020/07/06Room:4-ARack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Atg5autophagy-related 5 (yeast)10Atg5<tm1Myok>targeted mutation 1, Minesuke Yokoyama

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)10neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAPhage P1 LoxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E.coli neo, mouse Atg5 genome DNA
Research applicationCell Biology Research
Cre/loxP system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 441, 885-889 (2006).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorNoboru Mizushima (The University of Tokyo)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Mouse of the Month Jul 2009
Genetic Background
Breeding characters
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Lee TY, Lu WJ, Changou CA, Hsiung YC, Trang NTT, Lee CY, Chang TH, Jayakumar T, Hsieh CY, Yang CH, Chang CC, Chen RJ, Sheu JR, Lin KH.
Platelet autophagic machinery involved in thrombosis through a novel linkage of AMPK-MTOR to sphingolipid metabolism.
Autophagy (2021) 33749503

Mizushima N, Yoshimori T, Levine B.
Methods in mammalian autophagy research.
Cell 140(3) 313-26(2010) 20144757

Peng X, Wang Y, Li H, Fan J, Shen J, Yu X, Zhou Y, Mao H.
ATG5-mediated autophagy suppresses NF-κB signaling to limit epithelial inflammatory response to kidney injury.
Cell Death Dis 10(4) 253(2019) 30874544

Li K, Li M, Li W, Yu H, Sun X, Zhang Q, Li Y, Li X, Li Y, Abel ED, Wu Q, Chen H.
Airway epithelial regeneration requires autophagy and glucose metabolism.
Cell Death Dis 10(12) 875(2019) 31748541

Akoumianaki T, Kyrmizi I, Valsecchi I, Gresnigt MS, Samonis G, Drakos E, Boumpas D, Muszkieta L, Prevost MC, Kontoyiannis DP, Chavakis T, Netea MG, van de Veerdonk FL, Brakhage AA, El-Benna J, Beauvais A, Latge JP, Chamilos G.
Aspergillus Cell Wall Melanin Blocks LC3-Associated Phagocytosis to Promote Pathogenicity.
Cell Host Microbe 19(1) 79-90(2016) 26749442

Shroff A, Sequeira R, Patel V, Reddy KVR.
Knockout of autophagy gene, ATG5 in mice vaginal cells abrogates cytokine response and pathogen clearance during vaginal infection of Candida albicans.
Cell Immunol 324 59-73(2018) 29306553

Tang AH, Rando TA.
Induction of autophagy supports the bioenergetic demands of quiescent muscle stem cell activation.
EMBO J 33(23) 2782-97(2014) 25316028

Kurashige T, Nakajima Y, Shimamura M, Matsuyama M, Yamada M, Nakashima M, Nagayama Y.
Basal Autophagy Deficiency Causes Thyroid Follicular Epithelial Cell Death in Mice.
Endocrinology 160(9) 2085-2092(2019) 31314096

Bankston AN, Forston MD, Howard RM, Andres KR, Smith AE, Ohri SS, Bates ML, Bunge MB, Whittemore SR.
Autophagy is essential for oligodendrocyte differentiation, survival, and proper myelination.
Glia 67(9) 1745-1759(2019) 31162728

Alissafi T, Hatzioannou A, Mintzas K, Barouni RM, Banos A, Sormendi S, Polyzos A, Xilouri M, Wielockx B, Gogas H, Verginis P.
Autophagy orchestrates the regulatory program of tumor-associated myeloid-derived suppressor cells.
J Clin Invest 128(9) 3840-3852(2018) 29920188

Shen L, Yang Y, Ou T, Key CC, Tong SH, Sequeira RC, Nelson JM, Nie Y, Wang Z, Boudyguina E, Shewale SV, Zhu X.
Dietary PUFAs attenuate NLRP3 inflammasome activation via enhancing macrophage autophagy.
J Lipid Res 58(9) 1808-1821(2017) 28729463

Zhang Y, Cross SD, Stanton JB, Marmorstein AD, Le YZ, Marmorstein LY.
Early AMD-like defects in the RPE and retinal degeneration in aged mice with RPE-specific deletion of Atg5 or Atg7.
Mol Vis 23 228-241(2017) 28465655

Shen H, Zhu H, Panja D, Gu Q, Li Z.
Autophagy controls the induction and developmental decline of NMDAR-LTD through endocytic recycling.
Nat Commun 11(1) 2979(2020) 32532981

Eisenberg T, Abdellatif M, Schroeder S, Primessnig U, Stekovic S, Pendl T, Harger A, Schipke J, Zimmermann A, Schmidt A, Tong M, Ruckenstuhl C, Dammbrueck C, Gross AS, Herbst V, Magnes C, Trausinger G, Narath S, Meinitzer A, Hu Z, Kirsch A, Eller K, Carmona-Gutierrez D, Büttner S, Pietrocola F, Knittelfelder O, Schrepfer E, Rockenfeller P, Simonini C, Rahn A, Horsch M, Moreth K, Beckers J, Fuchs H, Gailus-Durner V, Neff F, Janik D, Rathkolb B, Rozman J, de Angelis MH, Moustafa T, Haemmerle G, Mayr M, Willeit P, von Frieling-Salewsky M, Pieske B, Scorrano L, Pieber T, Pechlaner R, Willeit J, Sigrist SJ, Linke WA, Mühlfeld C, Sadoshima J, Dengjel J, Kiechl S, Kroemer G, Sedej S, Madeo F.
Cardioprotection and lifespan extension by the natural polyamine spermidine.
Nat Med 22(12) 1428-1438(2016) 27841876

Torisu T, Torisu K, Lee IH, Liu J, Malide D, Combs CA, Wu XS, Rovira II, Fergusson MM, Weigert R, Connelly PS, Daniels MP, Komatsu M, Cao L, Finkel T.
Autophagy regulates endothelial cell processing, maturation and secretion of von Willebrand factor.
Nat Med 19(10) 1281-7(2013) 24056772

Rosenfeldt MT, O'Prey J, Morton JP, Nixon C, MacKay G, Mrowinska A, Au A, Rai TS, Zheng L, Ridgway R, Adams PD, Anderson KI, Gottlieb E, Sansom OJ, Ryan KM.
p53 status determines the role of autophagy in pancreatic tumour development.
Nature 504(7479) 296-300(2013) 24305049

Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht MT, Krause E, Maritzen T, Schmitz D, Haucke V.
Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
Neuron 109(2) 299-313.e9(2021) 33157003

Luo L, Ambrozkiewicz MC, Benseler F, Chen C, Dumontier E, Falkner S, Furlanis E, Gomez AM, Hoshina N, Huang WH, Hutchison MA, Itoh-Maruoka Y, Lavery LA, Li W, Maruo T, Motohashi J, Pai EL, Pelkey KA, Pereira A, Philips T, Sinclair JL, Stogsdill JA, Traunmüller L, Wang J, Wortel J, You W, Abumaria N, Beier KT, Brose N, Burgess HA, Cepko CL, Cloutier JF, Eroglu C, Goebbels S, Kaeser PS, Kay JN, Lu W, Luo L, Mandai K, McBain CJ, Nave KA, Prado MAM, Prado VF, Rothstein J, Rubenstein JLR, Saher G, Sakimura K, Sanes JR, Scheiffele P, Takai Y, Umemori H, Verhage M, Yuzaki M, Zoghbi HY, Kawabe H, Craig AM.
Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines: Prevalence of Germline Recombination and Influencing Factors.
Neuron 106(1) 37-65.e5(2020) 32027825

Sekiguchi K, Miyahara H, Inoue M, Kiyota K, Sakai K, Hanada T, Ihara K.
Metabolome Characteristics of Liver Autophagy Deficiency under Starvation Conditions in Infancy.
Nutrients 13(9) (2021) 34578904

Abernathy E, Mateo R, Majzoub K, van Buuren N, Bird SW, Carette JE, Kirkegaard K.
Differential and convergent utilization of autophagy components by positive-strand RNA viruses.
PLoS Biol 17(1) e2006926(2019) 30608919

Li X, Wu J, Sun X, Wu Q, Li Y, Li K, Zhang Q, Li Y, Abel ED, Chen H.
Autophagy Reprograms Alveolar Progenitor Cell Metabolism in Response to Lung Injury.
Stem Cell Reports 14(3) 420-432(2020) 32059792

Jessop F, Hamilton RF, Rhoderick JF, Shaw PK, Holian A.
Autophagy deficiency in macrophages enhances NLRP3 inflammasome activity and chronic lung disease following silica exposure.
Toxicol Appl Pharmacol 309 101-10(2016) 27594529

Ni HM, Boggess N, McGill MR, Lebofsky M, Borude P, Apte U, Jaeschke H, Ding WX.
Liver-specific loss of Atg5 causes persistent activation of Nrf2 and protects against acetaminophen-induced liver injury.
Toxicol Sci 127(2) 438-50(2012) 22491424