Strain Data Sheet

RBRC02975

Strain Information

Image
BRC No.RBRC02975
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Atg5<tm1Myok>
Former Common nameAtg5flox
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Kenji Nakamura, Mitsubishi Kagaku Institute of Life Sciences and Noboru Mizushima, Tokyo Metropolitan Institute of Medical Science in 2003. The construct was electoroporated into CCE/EK.CCE ES cells derived from 129S/SvEv-Gpi1<c>. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionAtg5 floxed mice. Exon 3 of Atg5 gene was flanked by loxP sites containing neomycin resistant cassette. Autophagy is an intracellular degradation process by an autophagosome, which contains a portion of cytoplasm and subsequently degrades upon fusion with a lysosome. Autophagy is considered to be important for the cellular response to starvation and the normal turnover of cytoplasmic components as well. Atg5, autophagy-related 5 is essential for autophagosome formation. Conditional B6.129S-Atg5<tm1Myok> (RBRC02975) mice can be crossed with various tissue-specific Cre transgenic mice to study the role of autophagy in adult tissues. Null knockout mice are also available: B6;129-Atg5<tm1Nmz> (RBRC02231).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJmsSlc]
References
Suppression of basal autophagy in neural cells causes neurodegenerative disease in mice.
Hara T, Nakamura K, Matsui M, Yamamoto A, Nakahara Y, Suzuki-Migishima R, Yokoyama M, Mishima K, Saito I, Okano H, Mizushima N
Nature, 441, 885-889 (2006). 16625204

Health Report

Examination Date / Room / Rack2024/11/18Room:4-ARack:B
2024/08/19Room:4-ARack:B
2024/05/20Room:4-ARack:B
2024/02/19Room:4-ARack:B
2023/11/20Room:4-ARack:B
2023/08/21Room:4-ARack:B
2023/06/26Room:4-ARack:B
2023/05/22Room:4-ARack:B
2023/02/20Room:4-ARack:B

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Atg5
MGI:1277186
autophagy related 510Atg5<tm1Myok>
MGI:3663625
targeted mutation 1, Minesuke Yokoyama
  • spinocerebellar ataxia, autosomal recessive 25(MedGEN)
  • loxP phage P1 loxP10loxP
    loxP phage P1 loxP10loxP
    neo neomycin resistance gene (E. coli)10 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • Purkinje cell degeneration(MP:0000876)

  • abnormal Paneth cell morphology(MP:0004843)

  • abnormal autophagy(MP:0008260)

  • abnormal axon morphology(MP:0005404)

  • abnormal behavior(MP:0004924)
  • more 36 phenotypes
  • abnormal brain morphology(MP:0002152)

  • abnormal cell physiology(MP:0005621)

  • abnormal cerebellar granule cell morphology(MP:0004098)

  • abnormal cerebral cortex morphology(MP:0000788)

  • abnormal hippocampus morphology(MP:0000807)

  • abnormal inferior colliculus morphology(MP:0000776)

  • abnormal liver morphology(MP:0000598)

  • abnormal motor capabilities/coordination/movement(MP:0002066)

  • abnormal proximal convoluted tubule morphology(MP:0004756)

  • abnormal suckling behavior(MP:0001436)

  • abnormal thalamus morphology(MP:0000832)

  • adipose tissue phenotype(MP:0005375)

  • aminoaciduria(MP:0003311)

  • ataxia(MP:0001393)

  • cellular phenotype(MP:0005384)

  • decreased a-wave amplitude(MP:0012143)

  • decreased b-wave amplitude(MP:0012144)

  • decreased body weight(MP:0001262)

  • decreased cerebral cortex pyramidal cell number(MP:0009974)

  • decreased grip strength(MP:0010053)

  • growth/size/body region phenotype(MP:0005378)

  • homeostasis/metabolism phenotype(MP:0005376)

  • hyperthreoninuria(MP:0020406)

  • impaired coordination(MP:0001405)

  • increased kidney weight(MP:0003917)

  • increased urine glucose level(MP:0001759)

  • limb grasping(MP:0001513)

  • mortality/aging(MP:0010768)

  • nervous system phenotype(MP:0003631)

  • neuron degeneration(MP:0003224)

  • neuronal intranuclear inclusions(MP:0004191)

  • postnatal growth retardation(MP:0001732)

  • premature death(MP:0002083)

  • short stride length(MP:0001407)

  • tremors(MP:0000745)

  • vision/eye phenotype(MP:0005391)
  • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 LoxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E.coli neo, mouse Atg5 genome DNA
    Research applicationCell Biology Research
    Cre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 441, 885-889 (2006). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorNoboru Mizushima (The University of Tokyo)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-
    Mouse of the Month Jul 2009

    BRC mice in Publications

    Sun F, Ali NN, Londoño-Vásquez D, Simintiras CA, Qiao H, Ortega MS, Agca Y, Takahashi M, Rivera RM, Kelleher AM, Sutovsky P, Patterson AL, Balboula AZ.
    Increased DNA damage in full-grown oocytes is correlated with diminished autophagy activation.
    Nat Commun 15(1) 9463(2024) 39487138
    Keary KM 3rd, Gu QH, Chen J, Li Z.
    Dendritic distribution of autophagosomes underlies pathway-selective induction of LTD.
    Cell Rep 42(8) 112898(2023) 37516958
    Yi-Qun Zhang, Ta Xiao, Chang-Jun Song, Yang-Ying Ke, Xiang Gao, Min Li, Heng Gu, Xu Chen
    Deficiency of Autophagy-Related Gene 5 in Keratinocytes Leads to Aggravation of Epidermal Damage in 2,4-dinitrochlorobenzene-induced Allergic Contact Dermatitis
    International Journal of Dermatology and Venereology Publish Ahead of Print (2023)
    Mitsui K, Hishiyama S, Jain A, Kotoda Y, Abe M, Matsukawa T, Kotoda M.
    Role of macrophage autophagy in postoperative pain and inflammation in mice.
    J Neuroinflammation 20(1) 102(2023) 37131209
    Minami Y, Hoshino A, Higuchi Y, Hamaguchi M, Kaneko Y, Kirita Y, Taminishi S, Nishiji T, Taruno A, Fukui M, Arany Z, Matoba S.
    Liver lipophagy ameliorates nonalcoholic steatohepatitis through extracellular lipid secretion.
    Nat Commun 14(1) 4084(2023) 37443159
    Cheng J, Wang Y, Yin L, Liang W, Zhang J, Ma C, Zhang Y, Liu B, Wang J, Zhao W, Li M, Wei L.
    The nonstructural protein 1 of respiratory syncytial virus hijacks host mitophagy as a novel mitophagy receptor to evade the type I IFN response in HEp-2 cells.
    mBio e0148023(2023) 37909764
    Suzuki Y, Aono Y, Akiyama N, Horiike Y, Naoi H, Horiguchi R, Shibata K, Hozumi H, Karayama M, Furuhashi K, Enomoto N, Fujisawa T, Nakamura Y, Inui N, Suda T.
    Involvement of autophagy in exacerbation of eosinophilic airway inflammation in a murine model of obese asthma.
    Autophagy 18(9) 2216-2228(2022) 35098856
    Yu S, Wang H, Liu M, Pei F, Liu H, Zhang J, Zhang L, Li Q, Chen Z.
    Loss of ATG5 in KRT14+ cells leads to accumulated functional impairments of salivary glands via pyroptosis.
    FASEB J 36(12) e22631(2022) 36342387
    Lee TY, Lu WJ, Changou CA, Hsiung YC, Trang NTT, Lee CY, Chang TH, Jayakumar T, Hsieh CY, Yang CH, Chang CC, Chen RJ, Sheu JR, Lin KH.
    Platelet autophagic machinery involved in thrombosis through a novel linkage of AMPK-MTOR to sphingolipid metabolism.
    Autophagy 17(12) 4141-4158(2021) 33749503
    Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht MT, Krause E, Maritzen T, Schmitz D, Haucke V.
    Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
    Neuron 109(2) 299-313.e9(2021) 33157003
    Sekiguchi K, Miyahara H, Inoue M, Kiyota K, Sakai K, Hanada T, Ihara K.
    Metabolome Characteristics of Liver Autophagy Deficiency under Starvation Conditions in Infancy.
    Nutrients 13(9) (2021) 34578904
    Shen H, Zhu H, Panja D, Gu Q, Li Z.
    Autophagy controls the induction and developmental decline of NMDAR-LTD through endocytic recycling.
    Nat Commun 11(1) 2979(2020) 32532981
    Luo L, Ambrozkiewicz MC, Benseler F, Chen C, Dumontier E, Falkner S, Furlanis E, Gomez AM, Hoshina N, Huang WH, Hutchison MA, Itoh-Maruoka Y, Lavery LA, Li W, Maruo T, Motohashi J, Pai EL, Pelkey KA, Pereira A, Philips T, Sinclair JL, Stogsdill JA, Traunmüller L, Wang J, Wortel J, You W, Abumaria N, Beier KT, Brose N, Burgess HA, Cepko CL, Cloutier JF, Eroglu C, Goebbels S, Kaeser PS, Kay JN, Lu W, Luo L, Mandai K, McBain CJ, Nave KA, Prado MAM, Prado VF, Rothstein J, Rubenstein JLR, Saher G, Sakimura K, Sanes JR, Scheiffele P, Takai Y, Umemori H, Verhage M, Yuzaki M, Zoghbi HY, Kawabe H, Craig AM.
    Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines: Prevalence of Germline Recombination and Influencing Factors.
    Neuron 106(1) 37-65.e5(2020) 32027825
    Li X, Wu J, Sun X, Wu Q, Li Y, Li K, Zhang Q, Li Y, Abel ED, Chen H.
    Autophagy Reprograms Alveolar Progenitor Cell Metabolism in Response to Lung Injury.
    Stem Cell Reports 14(3) 420-432(2020) 32059792
    Peng X, Wang Y, Li H, Fan J, Shen J, Yu X, Zhou Y, Mao H.
    ATG5-mediated autophagy suppresses NF-κB signaling to limit epithelial inflammatory response to kidney injury.
    Cell Death Dis 10(4) 253(2019) 30874544
    Li K, Li M, Li W, Yu H, Sun X, Zhang Q, Li Y, Li X, Li Y, Abel ED, Wu Q, Chen H.
    Airway epithelial regeneration requires autophagy and glucose metabolism.
    Cell Death Dis 10(12) 875(2019) 31748541
    Kurashige T, Nakajima Y, Shimamura M, Matsuyama M, Yamada M, Nakashima M, Nagayama Y.
    Basal Autophagy Deficiency Causes Thyroid Follicular Epithelial Cell Death in Mice.
    Endocrinology 160(9) 2085-2092(2019) 31314096
    Bankston AN, Forston MD, Howard RM, Andres KR, Smith AE, Ohri SS, Bates ML, Bunge MB, Whittemore SR.
    Autophagy is essential for oligodendrocyte differentiation, survival, and proper myelination.
    Glia 67(9) 1745-1759(2019) 31162728
    Chai Q, Wang X, Qiang L, Zhang Y, Ge P, Lu Z, Zhong Y, Li B, Wang J, Zhang L, Zhou D, Li W, Dong W, Pang Y, Gao GF, Liu CH.
    A Mycobacterium tuberculosis surface protein recruits ubiquitin to trigger host xenophagy.
    Nat Commun 10(1) 1973(2019) 31036822
    Abernathy E, Mateo R, Majzoub K, van Buuren N, Bird SW, Carette JE, Kirkegaard K.
    Differential and convergent utilization of autophagy components by positive-strand RNA viruses.
    PLoS Biol 17(1) e2006926(2019) 30608919
    Shroff A, Sequeira R, Patel V, Reddy KVR.
    Knockout of autophagy gene, ATG5 in mice vaginal cells abrogates cytokine response and pathogen clearance during vaginal infection of Candida albicans.
    Cell Immunol 324 59-73(2018) 29306553
    Alissafi T, Hatzioannou A, Mintzas K, Barouni RM, Banos A, Sormendi S, Polyzos A, Xilouri M, Wielockx B, Gogas H, Verginis P.
    Autophagy orchestrates the regulatory program of tumor-associated myeloid-derived suppressor cells.
    J Clin Invest 128(9) 3840-3852(2018) 29920188
    Shen L, Yang Y, Ou T, Key CC, Tong SH, Sequeira RC, Nelson JM, Nie Y, Wang Z, Boudyguina E, Shewale SV, Zhu X.
    Dietary PUFAs attenuate NLRP3 inflammasome activation via enhancing macrophage autophagy.
    J Lipid Res 58(9) 1808-1821(2017) 28729463
    Zhang Y, Cross SD, Stanton JB, Marmorstein AD, Le YZ, Marmorstein LY.
    Early AMD-like defects in the RPE and retinal degeneration in aged mice with RPE-specific deletion of Atg5 or Atg7.
    Mol Vis 23 228-241(2017) 28465655
    Akoumianaki T, Kyrmizi I, Valsecchi I, Gresnigt MS, Samonis G, Drakos E, Boumpas D, Muszkieta L, Prevost MC, Kontoyiannis DP, Chavakis T, Netea MG, van de Veerdonk FL, Brakhage AA, El-Benna J, Beauvais A, Latge JP, Chamilos G.
    Aspergillus Cell Wall Melanin Blocks LC3-Associated Phagocytosis to Promote Pathogenicity.
    Cell Host Microbe 19(1) 79-90(2016) 26749442
    Eisenberg T, Abdellatif M, Schroeder S, Primessnig U, Stekovic S, Pendl T, Harger A, Schipke J, Zimmermann A, Schmidt A, Tong M, Ruckenstuhl C, Dammbrueck C, Gross AS, Herbst V, Magnes C, Trausinger G, Narath S, Meinitzer A, Hu Z, Kirsch A, Eller K, Carmona-Gutierrez D, Büttner S, Pietrocola F, Knittelfelder O, Schrepfer E, Rockenfeller P, Simonini C, Rahn A, Horsch M, Moreth K, Beckers J, Fuchs H, Gailus-Durner V, Neff F, Janik D, Rathkolb B, Rozman J, de Angelis MH, Moustafa T, Haemmerle G, Mayr M, Willeit P, von Frieling-Salewsky M, Pieske B, Scorrano L, Pieber T, Pechlaner R, Willeit J, Sigrist SJ, Linke WA, Mühlfeld C, Sadoshima J, Dengjel J, Kiechl S, Kroemer G, Sedej S, Madeo F.
    Cardioprotection and lifespan extension by the natural polyamine spermidine.
    Nat Med 22(12) 1428-1438(2016) 27841876
    Jessop F, Hamilton RF, Rhoderick JF, Shaw PK, Holian A.
    Autophagy deficiency in macrophages enhances NLRP3 inflammasome activity and chronic lung disease following silica exposure.
    Toxicol Appl Pharmacol 309 101-10(2016) 27594529
    Phillip T Newton, Karuna K Vuppalapati, Thibault Bouderlique, Andrei S Chagin
    Pharmacological inhibition of lysosomes activates the MTORC1 signaling pathway in chondrocytes in an autophagy-independent manner
    Autophagy 11 1594-1607(2015)
    Tang AH, Rando TA.
    Induction of autophagy supports the bioenergetic demands of quiescent muscle stem cell activation.
    EMBO J 33(23) 2782-97(2014) 25316028
    Torisu T, Torisu K, Lee IH, Liu J, Malide D, Combs CA, Wu XS, Rovira II, Fergusson MM, Weigert R, Connelly PS, Daniels MP, Komatsu M, Cao L, Finkel T.
    Autophagy regulates endothelial cell processing, maturation and secretion of von Willebrand factor.
    Nat Med 19(10) 1281-7(2013) 24056772
    Rosenfeldt MT, O'Prey J, Morton JP, Nixon C, MacKay G, Mrowinska A, Au A, Rai TS, Zheng L, Ridgway R, Adams PD, Anderson KI, Gottlieb E, Sansom OJ, Ryan KM.
    p53 status determines the role of autophagy in pancreatic tumour development.
    Nature 504(7479) 296-300(2013) 24305049
    Ni HM, Boggess N, McGill MR, Lebofsky M, Borude P, Apte U, Jaeschke H, Ding WX.
    Liver-specific loss of Atg5 causes persistent activation of Nrf2 and protects against acetaminophen-induced liver injury.
    Toxicol Sci 127(2) 438-50(2012) 22491424
    Mizushima N, Yoshimori T, Levine B.
    Methods in mammalian autophagy research.
    Cell 140(3) 313-26(2010) 20144757