RBRC02958

Information1

Image
BRC No.RBRC02958
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(tetO-EGFP/TeNT)1Nak
Former Common nameTeNT
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJmsSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Mutsuya Yamamoto and Shigetada Nakanishi in 2003.
Strain descriptionThis transgenic mice express the EGFP and tetanus neurotoxin light chain gene under the control of the tetracycline operator sequence.
Colony maintenanceHemizygote x C57BL/6 [C57BL/6JJmsSlc]
ReferencesJ. Neurosci., 23, 6759-6767 (2003). 12890769

Health Report

Examination Date / Room / Rack2012/04/16Room:3-DRack:A
2012/02/29Room:3-DRack:A
2012/02/27Room:3-DRack:A
2012/02/27Room:3-DRack:A
2012/02/20Room:3-DRack:A
2012/02/08Room:3-DRack:A
2011/12/12Room:3-DRack:A
2011/12/12Room:3-DRack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Odc1mouse ornithine decarboxylase (PEST sequence) cDNAUNOdc1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNE. coli bidirectional TRE promoter (tetracycline responsive promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
TeNTtetanus neurotoxin light chain (Clostridium tetani)UNTeNT

Information2

Donor DNATetracycline response element-CMV promoter, Aequorea victoria EGFP, Clostridium tetani TeNT light chain, mouse PEST sequence (proline, glutamic acid, serine, and threonine) of ornithine decarboxylase (residues 1402-1524), rabbit beta-globin poly A signal
Research applicationFluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 23, 6759-6767 (2003). Proc. Natl. Acad. Sci. USA, 104, 16690-16695 (2007).The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorShigetada Nakanishi (Osaka Bioscience Institute)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

Yasutaka Niwa, Genki N. Kanda, Rikuhiro G. Yamada, Shoi Shi, Genshiro A. Sunagawa,Maki Ukai-Tadenuma, Hiroshi Fujishima, Naomi Matsumoto, Koh-hei Masumoto, Mamoru Nagano,Takeya Kasukawa, James Galloway, Dimitri Perrin, Yasufumi Shigeyoshi, Hideki Ukai, Hiroshi Kiyonari,Kenta Sumiyama, and Hiroki R. Ueda.
Muscarinic Acetylcholine Receptors Chrm1 and Chrm3 Are Essential for REM Sleep.
Cell Rep 2231-2247(2018) 30157420