Strain Data Sheet

RBRC02957

Strain Information

Image
BRC No.RBRC02957
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Gabra6-rtTA)1Nak
Former Common nameTeT
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJmsSlc
Appearance
Strain developmentDeveloped by Mutsuya Yamamoto and Shigetada Nakanishi in 2003.
Strain description
Colony maintenance
References
Conditioned eyeblink learning is formed and stored without cerebellar granule cell transmission.
Wada N, Kishimoto Y, Watanabe D, Kano M, Hirano T, Funabiki K, Nakanishi S
Proc. Natl. Acad. Sci. USA, 104, 16690-16695 (2007). 17923666

Reversible suppression of glutamatergic neurotransmission of cerebellar granule cells in vivo by genetically manipulated expression of tetanus neurotoxin light chain.
Yamamoto M, Wada N, Kitabatake Y, Watanabe D, Anzai M, Yokoyama M, Teranishi Y, Nakanishi S
J. Neurosci., 23, 6759-6767 (2003). 12890769

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
IRESinternal ribosomal entry site (EMCV)UN
rtTAE. coli Reverse tetracycline transactivator (Mutated tetrepressor DNA-binding domain and the transactivation domain of VP-16)UNrtTAmouse GABAa receptor 6 subunit promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse GABAa receptor 6 subunit promoter, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), rtTA[E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain], SV40 polyA signal
    Research applicationTet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 23, 6759-6767 (2003). Proc. Natl. Acad. Sci. USA, 104, 16690-16695 (2007).The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorShigetada Nakanishi (Osaka Bioscience Institute)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data