Strain Data Sheet

RBRC02951

Strain Information

Image
BRC No.RBRC02951
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Cacnb3<tm1Acv>
Former Common nameccb3 null
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Pain perception in mice lacking the beta3 subunit of voltage-activated calcium channels.
Murakami M, Fleischmann B, De Felipe C, Freichel M, Trost C, Ludwig A, Wissenbach U, Schwegler H, Hofmann F, Hescheler J, Flockerzi V, Cavalie A
J. Biol. Chem., 277, 40342-40351 (2002). 12161429

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cacnb3calcium channel, voltage-dependent, beta 3 subunit15Cacnb3targeted mutation 1, Adolfo Cavalie
neoneomycin resistance gene (E. coli)15herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal CD4-positive, alpha-beta T cell physiology(MP:0005463)

  • abnormal calcium ion homeostasis(MP:0004231)

  • abnormal interleukin secretion(MP:0008568)

  • abnormal vascular smooth muscle morphology(MP:0005592)

  • abnormal vascular smooth muscle physiology(MP:0005595)
    • more 7 phenotypes
  • decreased interferon-gamma secretion(MP:0008567)

  • enlarged heart(MP:0000274)

  • increased heart weight(MP:0002833)

  • increased systemic arterial blood pressure(MP:0002842)

  • increased systemic arterial diastolic blood pressure(MP:0006143)

  • increased systemic arterial systolic blood pressure(MP:0006144)

  • vascular smooth muscle hypertrophy(MP:0002975)
    • Detailed phenotype data

    Ordering Information

    Donor DNACacnb3 cDNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neomycin resistance gene, mouse Cacnb3 genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR.
    DepositorManabu Murakami (Akita University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data