Strain Data Sheet

RBRC02948

Strain Information

Image
BRC No.RBRC02948
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Cadps2<tm1Tfr>
Former Common nameCAPS2 KO
H-2 Haplotype
ES Cell lineMS12 [C57BL/6]
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
References
CAPS2 Deficiency Impairs the Release of the Social Peptide Oxytocin, as Well as Oxytocin-Associated Social Behavior.
Shuhei Fujima, Ryosuke Yamaga, Haruka Minami, Shota Mizuno, Yo Shinoda, Tetsushi Sadakata, Manabu Abe, Kenji Sakimura, Yoshitake Sano, Teiichi Furuichi
J. Neurosci., 41(20):4524-4535 (2021). 33846232

Impaired cerebellar development and function in mice lacking CAPS2, a protein involved in neurotrophin release.
Sadakata T, Kakegawa W, Mizoguchi A, Washida M, Katoh-Semba R, Shutoh F, Okamoto T, Nakashima H, Kimura K, Tanaka M, Sekine Y, Itohara S, Yuzaki M, Nagao S, Furuichi T
J. Neurosci., 27, 2472-2482 (2007). 17344385

Autistic-like phenotypes in Cadps2-knockout mice and aberrant CADPS2 splicing in autistic patients.
Sadakata T, Washida M, Iwayama Y, Shoji S, Sato Y, Ohkura T, Katoh-Semba R, Nakajima M, Sekine Y, Tanaka M, Nakamura K, Iwata Y, Tsuchiya K J, Mori N, Detera-Wadleigh S D, Ichikawa H, Itohara S, Yoshikawa T, Furuichi T
J. Clin. lnvest., 117, 931-943 (2007) . 17380209

CAPS2 Deficiency Impairs the Release of the Social Peptide Oxytocin, as Well as Oxytocin-Associated Social Behavior.
Fujima S, Yamaga R, Minami H, Mizuno S, Shinoda Y, Sadakata T, Abe M, Sakimura K, Sano Y, Furuichi T
J. Neurosci., 41(20):4524-4535 (2021). 33846232

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cadps2Ca2+-dependent activator protein for secretion 26Cadps2targeted mutation 1, Teiichi Furuichi
loxPphage P1 loxP6loxP
loxPphage P1 loxP6loxP
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal Purkinje cell dendrite morphology(MP:0008572)

  • abnormal brain interneuron morphology(MP:0004101)

  • abnormal brain morphology(MP:0002152)

  • abnormal cerebellar granule cell migration(MP:0009977)

  • abnormal cerebellum external granule cell layer morphology(MP:0000872)
    • more 19 phenotypes
  • abnormal cerebellum fissure morphology(MP:0009267)

  • abnormal cerebellum morphology(MP:0000849)

  • abnormal circadian behavior(MP:0020467)

  • abnormal emotion/affect behavior(MP:0002572)

  • abnormal maternal nurturing(MP:0001386)

  • abnormal neuron specification(MP:0002948)

  • abnormal optokinetic reflex(MP:0009619)

  • abnormal paired-pulse facilitation(MP:0002918)

  • abnormal response to novel object(MP:0003461)

  • abnormal sleep pattern(MP:0001501)

  • abnormal social investigation(MP:0001360)

  • abnormal social/conspecific interaction behavior(MP:0002557)

  • abnormal spatial reference memory(MP:0008414)

  • decreased Purkinje cell number(MP:0000880)

  • decreased body weight(MP:0001262)

  • decreased exploration in new environment(MP:0001417)

  • hyperactivity(MP:0001399)

  • impaired coordination(MP:0001405)

  • increased anxiety-related response(MP:0001363)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 phage loxP sites, mouse Phosphoglycerate kinase promoter (PGK promoter), E.coli neo resistance gene, SV40 polyA signal, mouse Cadps2 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsThe BIOLOGICAL RESOURCE shall be used for non-profit research purpose. To avoid possible conflict of interest with the DEPOSITOR, the RECIPIENT of the BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research with the DEPOSITOR or a collaborative research requiring co-authorship of the DEPOSITOR for TWO years after receipt of the BIOLOGICAL RESOURCE. If no publication is made within the TWO years, the first publication shall be co-authored by the DEPOSITOR.
    DepositorTeiichi furuichi (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data