RBRC02945

Information1

Image
BRC No.RBRC02945
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Tg(Thy1-ECFP,-EYFP)1Hsak
Former Common nameThy1-stop-syn::ECFP-ires-gEYFP
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Ko Kobayakawa, Reiko Hayashi and Hitoshi Sakano, School of Science The University of Tokyo in 2002. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs.
Strain descriptionThy1-stop-syn::CFP-ires-gapEYFP mice having a transgene of a Thy1 promoter, loxP-STOP loxP cassette, synaptotagmin-CFP and ires-gapEYFP. Tissue-specific expression of Cre recombinase has not been examined yet.
Colony maintenanceCarrier x Noncarrier [or Crossing to Crlj:CD1(ICR)]
References

Health Report

Examination Date / Room / Rack2010/04/05Room:3-7Rack:B
2010/02/08Room:3-7Rack:B
2009/12/07Room:3-7Rack:B
2009/10/05Room:3-7Rack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
ECFPEnhanced Cyan Fluorescent Protein (Aequorea victoria), F64L/S65T/K212N/H231LUNECFPmouse Thy1 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)UNEYFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
His3His3 (S. cerevisiae)UNHis3

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
mouse gap43 palmitoylation signalUN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
mouse synaptotagmin I (Syt1)UN

Information2

Donor DNAMouse Thy1 promoter, P1 phage loxP site, S. cerevisiae His3, Jellyfish ECFP, EYFP, encephalomyocarditis virus ires (internal ribosome entry site, Mouse gap43 palmitoylation signal, Mouse synaptotagmin I (Syt1)
Research applicationCell Biology Research
Fluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER (Ko KOBAYAKAWA : skobayak@mail.ecc.u-tokyo.ac.jp). The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in first publication of the results obtained from the use of the BIOLOGICAL RESOURCE.
DepositorHitoshi Sakano (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data