Strain Data Sheet

RBRC02932

Information1

Image
BRC No.RBRC02932
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Olfr16-Olr226,-Cre,-EYFP)1Hsak
Former Common nameI7(WT)-IRES-Cre-IRES-EYFP, I7(WT)-Cre
H-2 Haplotype
ES Cell line
Background strainC57BL/6
Appearance
Strain development
Strain description
Colony maintenance
ReferencesScience, 314, 657-661 (2006). 16990513

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gap43growth associated protein 43UNGap43B-50, GAP-43, Gap43, neuromodulin

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)UNEYFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)UNFLAG

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HAhuman influenza virus HA (hemagglutinin) tag sequenceUNHA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)UNinternal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
iCrephage P1 codon improved Cre (iCre)UNiCre

Information2

Donor DNAMouse Olfr16, Rat Olfr226, encephalomyocarditis virus ires (internal ribosome entry site), Jellyfish EYFP cDNA, E. coli Cre recombinase gene, FLAG tag (synthetic), Human influenza virus Ha(hemagglutinin) tag, Mouse gap43 palmitoylation signal
Research applicationCell Biology Research
Cre/loxP system
Fluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 314, 657-661 (2006).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorHitoshi Sakano (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

Zapiec B, Bressel OC, Khan M, Walz A, Mombaerts P.
Neuropilin-1 and the Positions of Glomeruli in the Mouse Olfactory Bulb.
eNeuro 3(5) (2016) 27844052