Strain Data Sheet

RBRC02847

Information1

Image
BRC No.RBRC02847
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129S6/SvEvTac-Dkk3<tm1Tfur>
Former Common nameDKK3 K.O.
H-2 Haplotype
ES Cell lineTC1 [129S6/SvEvTac]
Background strain129S6/SvEvTac
Appearancelight-bellied agouti [A<w>/A<w> B/B C/C]
Strain developmentDeveloped by Dr. Takahisa Furukawa, Osaka Bioscience Institute in 2007.
Strain descriptionDkk3 knockout mice. Homozygous mutant mice show no obvious phenotype.
Colony maintenanceSibling Mating (Homozygote x Homozygote)
References

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dkk3dickkopf homolog 3 (Xenopus laevis)7Dkk3<tm1Tfur>targeted mutation 1, Takahisa Furukawadickkopf homolog 3 (Xenopus laevis)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signal7

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, SV40 pA, mouse Dkk3 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorTakahisa Furukawa (Osaka Bioscience Institute)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Habuta M, Fujita H, Sato K, Bando T, Inoue J, Kondo Y, Miyaishi S, Kumon H, Ohuchi H.
Dickkopf3 (Dkk3) is required for maintaining the integrity of secretory vesicles in the mouse adrenal medulla.
Cell Tissue Res 379(1) 157-167(2020) 31673758

Zhu Y, Demidov ON, Goh AM, Virshup DM, Lane DP, Bulavin DV.
Phosphatase WIP1 regulates adult neurogenesis and WNT signaling during aging.
J Clin Invest 124(7) 3263-73(2014) 24911145

Inoue J, Fujita H, Bando T, Kondo Y, Kumon H, Ohuchi H.
Expression analysis of Dickkopf-related protein 3 (Dkk3) suggests its pleiotropic roles for a secretory glycoprotein in adult mouse.
J Mol Histol 48(1) 29-39(2017) 27866302