RBRC02831

Information1

Image
BRC No.RBRC02831
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S6-Gcg<tm1Yhys>
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJmsSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Yoshitaka Hayashi, Nagoya University in 2007. Gcg gene was disrupted by introduction of GFP cDNA. GFP expresses in Gcg positive cells.
Strain descriptionGlucagon, Gcg deficient mice. Homozygous mutant mice lack peptides derived from proglucagon.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
ReferencesMol. Endocrinol., 23, 1990-1999 (2009). 19819987

Health Report

Examination Date / Room / Rack2011/04/18Room:3-DRack:E
2011/02/21Room:3-DRack:E
2010/12/13Room:3-DRack:E
2010/10/18Room:3-DRack:E
2010/08/23Room:3-DRack:E
2010/06/21Room:3-DRack:E
2010/04/19Room:3-DRack:E
2010/02/22Room:3-DRack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gcgglucagon2Gcg<tm1Yhys>transgene insertion 1, Yoshitaka Hayashi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAA. victoria Enhanced green fluorescent protein cDNA, Bovine Growth hormone poly adenylation signal, mouse phosphoglycerate kinase promoter, E. coli Aminoglycoside phosphotransferase cDNA (E.coli neo), mouse phosphoglycerate kinase poly adenylation signal, mouse Gcg genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorYoshitaka Hayashi (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Mouse of the Month Nov 2012

BRC mice in Publications

Fukami A, Seino Y, Ozaki N, Yamamoto M, Sugiyama C, Sakamoto-Miura E, Himeno T, Takagishi Y, Tsunekawa S, Ali S, Drucker DJ, Murata Y, Seino Y, Oiso Y, Hayashi Y.
Ectopic Expression of GIP in Pancreatic beta-Cells Maintains Enhanced Insulin Secretion in Mice With Complete Absence of Proglucagon-Derived Peptides.
Diabetes 62(2) 510-8(2013) 23099862