RBRC02821

Information1

Image
BRC No.RBRC02821
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Gab2<tm1Thir>
Former Common nameGab2 KO mouse
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6 and 129 mixed
Appearanceback or agouti (not fixed yet)
Strain developmentDeveloped by Toshio Hirano, Osaka University Graduate School of Medicine in 2002. R1 ES cells were used. C57BL/6 and 129 mixed background.
Strain descriptionGab2 gene knockout mice. Exon encoding a major portion of the PH domain was replaced with a neo cassette. Gab2 gene is an adaptor molecule that plays a critical role in mediating cellular transformation. The number of mast cells is severely reduced in the stomach and less severely in the skin of homozygous mutant mice. Bone marrow-derived mast cells from the homozygous mutant mice exhibit an impaired KitL-induced ERK MAP kinase and Akt activation.
Colony maintenanceHeterozygote x Homozygote [or Crossing to C57BL/6JJcl] Reproductive performance is not so good.
ReferencesBlood, 99 1866-1869 (2002). 11861309

Health Report

Examination Date / Room / Rack2013/08/05Room:3-5Rack:D
2013/06/03Room:3-5Rack:D
2013/04/01Room:3-5Rack:D
2013/02/04Room:3-5Rack:D
2012/12/03Room:3-5Rack:D
2012/10/01Room:3-5Rack:D
2012/08/06Room:3-5Rack:D
2012/04/02Room:3-5Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gab2growth factor receptor bound protein 2-associated protein 27Gab2<tm1Thir>targeted mutation 1, Toshio Hirano

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAE. coli Neomycin resistance gene, mouse phosphoglycerate kinase promoter, mouse Gab2 genomic DNA
Research applicationCell Biology Research
Immunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Blood, 99, 1866-1869 (2002).The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECPIENT who wants to use the BIOLOGICAL RESOURCE for the purpose other than education and research in non-profit organization is requested to make a Material Transfer Agreement with Osaka University.
DepositorToshio Hirano (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data