RBRC02811

Information1

Image
BRC No.RBRC02811
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain namePchdg-gHS4-5
Former Common namegHS4-5
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University. TT2 ES cells were used. The gHS4-5 dNeo mice were generated by crossing with gHS4-5 (RBRC02811) and FLP mice.
Strain descriptionPcdhg gene floxed mice. The gHS4-5 genomic region of the Pcdhg gene was flanked by loxP sites. Frt-neo cassette type (RBRC02811 and RBRC03155), delta neo cassette type (RBRC03153).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
ReferencesJ Biol Chem. , 286, 31885-31895 (2011). 21771796

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pcdhgprotocadherin gamma cluster18Pcdhg<tm(gHS4-5)>targeted mutation

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site18Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)18mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 loxP, yeast FRT (flipase recombination target) site, Transposon Tn5 Neo, mouse phosphoglycerate kinase promoter (PGK promoter), Mouse Pcdhg gene
Research applicationCre/loxP system
FLP/frt system
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from DEPOSITOR.
DepositorTakeshi Yagi (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP (Japanese)

BRC mice in Publications

No Data