RBRC02794

Information1

Image
BRC No.RBRC02794
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Vps52<tm1Kab>/95Rbrc
Former Common nameVps52 #95(Acc.No.CDB0767K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Michihiko Sugimoto and Kuniya Abe, RIKEN BioResource center in 2008. TT2 (B6CBF1 derived) ES cells were used to generate the targeted mice. C57BL/6 and CBA mixed (C57BL/6 semicongenic).
Strain descriptionVps52 2lox2frt (neo) targeted mice in which Frt-neo cassette (PGK-neo) was inserted upstream of exon 2, and two loxP sites were located in introns 1 and 2. Homozygous mutant mice were embryonic lethal. Line 95 (main line) (RBRC02794), Line 16 (sub line) (RBRC02832), Line 20 (sub line) (RBRC02857).
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
ReferencesCell Reports, 2, 1363-1374 (2012). 23142660

Health Report

Examination Date / Room / Rack2014/11/10Room:2-検Rack:E
2014/09/08Room:2-検Rack:E
2014/09/08Room:2-3Rack:E
2014/07/07Room:2-検Rack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Vps52VPS52 GARP complex subunit17Vps52<tm1Kab>targeted mutation 1, Kuniya Abe

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site17Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site17Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBB2rabbit beta-globin17HBB2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lox71mutant lox sequence17lox71

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)17mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAE. coli neo, Phage P1 lox71, Phage P1 loxP, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), mouse PGK 3’end, rabbit beta-globin 3' end
Research applicationCre/loxP system
Developmental Biology Research
FLP/frt system
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Dr. Kuniya Abe (abe@brc.riken.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0767K and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested (Cell Rep. 2012 2(5):1363-74.). (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Lab HP

BRC mice in Publications

No Data