Strain Data Sheet



BRC No.RBRC02781
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Cd8a<a> Tg(Tcra2C,Tcrb2C)1Dlo
Former Common name2C TCR, B6-Cd8a<a> 2CTCR Tg
H-2 Haplotype
ES Cell line
Background strain
Strain developmentThe transgene was developed by Dennis Loh, Washington University School of Medicine in 1988. The mice were deposited by Takanori Teshima, Kyushu University Hospital, through Paul Martin, Fred Hutchinson Cancer Research Center. H-2b and Ly2.1 (Cd8.1) background.
Strain description2C TCR transgenic mice expressing the alpha beta antigen receptor from the cytotoxic T-lymphocyte clone 2C. Ly2.1 (Cd8.1) congenic line.
Colony maintenanceTg : Carrier x Noncarrier ; Ly2 : Homozygote x Homozygote
ReferencesNature, 335, 271-274 (1988). 3261843
J. Immunol., 157, 4297-4308 (1996). 8906803

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cd8aCD8 antigen, alpha chain6Cd8a<a>a variant

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tcra2CT cell receptor alpha chainUNTcra2Ctransgene insertion 1, Dennis Y Loh

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tcrb2CT cell receptor beta chainUNTcrb2Ctransgene insertion 1, Dennis Y Loh

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)UN


Donor DNAmouse Functional rearranged TCR VJ alpha and VDJ beta genes from a Lambda Dash library of 2C DNA, mouse Cosmid C41/45 from Balb/c, mouse Cosmid containing C alpha complementary DNA from DBA/2 liver cosmid library, E. coli neo
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorTakanori Teshima (Kyushu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data