RBRC02771

Information1

Image
BRC No.RBRC02771
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC.B-17/Icr Prkdc<scid>-Tg(INS-TRECK/DTR)1Rin
Former Common nameBALB/c Prkdc<scid>-Tg(INS-TRECK/DTR)1Rin
H-2 Haplotype
ES Cell line
Background strainC.B-17/Icr-scid/Jcl
Appearance
Strain developmentDeveloped by Kunie Matsuoka and Hiromichi Yonekawa, Tokyo Metropolitan Institute of Medical Science. The transgene was injected into the pronuclei of C.B-17/Icr-scid fertilized eggs. RBRC02767 (line 5), RBRC02768 (line 14), RBRC02769 (line 17), RBRC02770 (line 33), RBRC02771 ((scid background, the same line as the #70 in BBRC2013 article).
Strain descriptionscid-INS-TRECK Transgenic mice, having the diphtheria toxin receptor (hDTR) driven by the human insulin promoter and Prkdc<scid> mutation. Hyperglycemia was observed within 3 days after the administration of DT.
Colony maintenance
ReferencesTransgenic Res., 21(1):51-62 (2011). 21431867
Biochem. Biophys. Res. Commun., 436, 400-405 (2013). 23747725

Health Report

Examination Date / Room / Rack2016/03/07Room:2-検Rack:F
2015/11/09Room:2-検Rack:G
2015/09/07Room:2-検Rack:G
2015/07/06Room:2-検Rack:G
2015/05/11Room:2-検Rack:G
2015/03/09Room:2-検Rack:G
2015/01/13Room:2-検Rack:G
2014/11/10Room:2-検Rack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Prkdcprotein kinase, DNA activated, catalytic polypeptide16Prkdc<scid>severe combined immunodeficiency

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBEGFheparin-binding EGF-like growth factor (human)UNHBEGFhuman Insulin promoter

Information2

Donor DNAHuman Insulin promoter (SphI/BamHI) fragment, Rabbit Beta-globin intron, Human HB-EGF cDNA, Rabbit Beta-globin polyA/regulatory domain
Research applicationDiabetes and Obesity Research
Immunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 436, 400-405 (2013).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT of BIOLOGICAL RESOURCE, who belongs to a profit organization must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorHiromichi Yonekawa (Tokyo Metropolitan Institute of Medical Science)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data