RBRC02770

Information1

Image
BRC No.RBRC02770
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(InsBAC-TRECK/DTR)33Rin
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Kunie Matsuoka and Hiromichi Yonekawa, Tokyo Metropolitan Institute of Medical Science. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs. RBRC02767 (line 5), RBRC02768 (line 14), RBRC02769 (line 17), RBRC02770 (line 33), RBRC02771 (scid background, the same line as the #70 in BBRC2013 article).
Strain descriptionINS2(BAC)-TRECK transgenic mice, expressing hDTR cDNA under the control of the mouse insulin II promoter. Hyperglycemia, severe ablation of pancreatic beta cells and depletion of serum insulin were observed within 3 days after the administration of DT.
Colony maintenance
ReferencesBiochem. Biophys. Res. Commun., 436, 400-405 (2013). 23747725
Transgenic Res., 21(1):51-62 (2011). 21431867

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBEGFheparin-binding EGF-like growth factor (human)UNHBEGFmouse Ins2 promoter

Information2

Donor DNAMouse RP23-92L23 BAC clone, Human HB-EGF cDNA, (The cording region of mouse ins2 derived from a 280 kb BAC clone is substituted human diphtheria toxin receptor (HB-EGF) cDNA by homologous recombination)
Research applicationDiabetes and Obesity Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 436, 400-405 (2013).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT of BIOLOGICAL RESOURCE, who belongs to a profit organization must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorHiromichi Yonekawa (Tokyo Metropolitan Institute of Medical Science)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data