RBRC02760

Information1

Image
BRC No.RBRC02760
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Atg7<tm1.1Tchi>
Former Common nameC57BL/6-Atg7+/-
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Komatsu and Chiba, Tokyo Metropolitan Institute of Medical Science.
Strain descriptionAtg7, autophagy-related 7 gene knock-out mice. Atg7 is essential for ATG conjugation, LC3 modification systems, and autophagosome formation. Homozygous mutant mice die within 1 day of delivery.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]Homozygous mutant mice die within 1 day after birth.
ReferencesJ. Cell Biol., 169, 425-434 (2005) 15866887

Health Report

Examination Date / Room / Rack2011/01/17Room:4-ARack:E
2010/11/16Room:4-ARack:E
2010/09/13Room:4-ARack:E
2010/07/12Room:4-ARack:E
2010/05/17Room:4-ARack:E
2010/03/15Room:4-ARack:E
2010/01/18Room:4-ARack:E
2009/11/16Room:4-ARack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Atg7autophagy-related 7 (yeast)6Atg7<tm1.1Tchi>targeted mutation 1.1, Tomoki Chiba

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Information2

Donor DNAPhage P1 loxP site, Mouse Atg7 genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Blol., 169, 425-434 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE, Who belongs to a profit organization must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasaaki Komatsu (Tokyo Metropolitan Institute of Medical Science)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-
Mouse of the Month Jun 2012, Mouse of the Month Oct 2016
Genetic Background

BRC mice in Publications

No Data