RBRC02717

Information1

Image
BRC No.RBRC02717
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(CAG-xstopx-Plce1,-lacZ)ATkat
Former Common nameCAG-flox-PLCε-A
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
ReferencesEur J Immunol., 41(1):202-213(2011). 21182091

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Plce1phospholipase C, epsilon 1 cDNAUNPlce1CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
poly ASV40 polyA signalUNpoly A

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Phage P1 loxP sites, STOP sequence contained a 550-bp C-terminal sequence of yeast His3 gene, mouse Plce1 cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Simian virus 40 Large T antigen nuclear localization signal (NLS), E.coli lacZ, SV40 polyA signal
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. European Journal of Immunology, 41, 202-213 (2011).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorTohru Kataoka (Kobe University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data