RBRC02716

Information1

Image
BRC No.RBRC02716
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129X1-Plce1<tm1Tkat>
Former Common namePLCεKO
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. T. Kataoka, N. Suzuki and H. Saito in 2001.
Strain descriptionPhospholipase Cepsilon is a novel class of phosphoinositide-specific phospholipase C that were identified as a downstream effector of Ras and Rap small GTPases. This knockout strain expesses a phospolipase Cepsilon catalytically inactivated by gene targeting. Homozygous mutant mice show a congenital semilunar valvulogenesis defect.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
ReferencesCancer Res., 15, 8808-8810 (2004) 15604236
Mol. Cell Biol., 25, 2191-2199 (2005) 15743817

Health Report

Examination Date / Room / Rack2009/05/19Room:4-ARack:C
2009/05/18Room:4-ARack:C
2009/03/16Room:4-ARack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Plce1phospholipase C, epsilon 119Plce1<tm1Tkat>targeted mutation 1, Tohru Kataoka

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)19mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, SV40 late gene polyA signal, mouse Plce1 genomic DNA
Research applicationCardiovascular Research
Mouse Models for Human Disease
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 25, 2191-2199 (2005). Cancer Res., 64, 8808-8810 (2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorTohru Kataoka (Kobe University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data