RBRC02712

Information1

Image
BRC No.RBRC02712
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gpr126<tm1Taki>
Former Common nameDREG Knock-out Mouse
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Akiyama T, The University of Tokyo.
Strain descriptionGpr129 knock-out mice. Gpr129, G-protein-coupled receptor 126 is a member of seven-transmembrane proteins related to family-B G-protein- coupled receptors (LNB-TM7), expresses at high levels in the heart and somites during mouse embryogenesis and in the adult lung. Homozygous mutant mice are embryonic lethal (E12.5).
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesGenes Cells, 9, 549-560 (2004) 15189448

Health Report

Examination Date / Room / Rack2008/10/20Room:3-CRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gpr126G protein-coupled receptor 12610Gpr126<tm1Taki>targeted mutation 1, Tetsu Akiyama

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)10

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)10herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAE. coli neo, E. coli lacZ, polyoma enhancer/herpes simplex virus thymidine kinase promoter (HSV tk promoter)(MC1), Phage P1 loxP sites
Research applicationDevelopmental Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells, 9, 549-560 (2004).The DEPOSITOR should be a co-author in articles when the RECIPIENTs publish their data using the BIOLOGICAL RESOURCE for two years after deposition to the RIKEN BRC. After two years from deposition, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorTetsu Akiyama (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data