RBRC02705

Information1

Image
BRC No.RBRC02705
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(FucciS/G2/M)#492Bsi
Former Common nameB6.Cg-Tg(Fucci)492Bsi, B6;B6D2-Tg(Fucci)492Bsi
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Asako Sakaue-Sawano and Atsushi Miyawaki, RIKEN Brain Science Institute. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6N.
Strain descriptionTransgenic mouse strains express fucci (fluorescent, ubiquitination-based cell cycle indicator) under the control of CAG promoter. These mice can be used to clearly visualize the cell cycle with fluorescence, i.e. S/G2/M phase nuclei green and those in G1 phase red in vivo. #474 (RBRC02704), #492 (RBRC02705), #504 (RBRC02706): Fucci-S/G2/M-Green (mAG-hGeminin(1/110)). #596 (RBRC02707), #610 (RBRC02708), #639 (RBRC02709): Fucci-G1-Red (mKO2-hCdt1(30/120)). For whole body (various tissues): #504 and #596 (Cell 2008). For hematopoietic system (monocyte and macrophage): #474 and #610 (Development 2013). For lymphocytic system: #492 and 639 (PNAS 2010). #474 and #610 double transgenic mice (RBRC02817). #504 and #596 double transgenic mice (RBRC02892). Whole-body visualization of Fucci mice with a flashlight (PCR genotyping is also available.): Check the Fucci S/2G/M-Green expressing mice at 1-4 days after birth by using blue LED flashlight through a sharp-cut filter in the dark box. Check the Fucci G1-Red expressing mice within 1-4 days after birth by using green LED flashlight through a green band-cut filter in the dark box. The part with few hairs such as in the mouth and auricles is suitable for the observation of fluorescence. Visualizing device: blue LED flashlight (470 nm), S470/30 filter, 535AF26 filter, sharp-cut filter (500 or 520 nm), green band-cut filter (red filter). #474 and #504: Tg homozygous mice are viable and fertile. #596: Tg homozygous mice are probably lethal (details unknown). #492, #610 and #639: Viability and fertility of homozygous mice are unknown (probably decreased fertility, details unknown).
Colony maintenanceCarrier x Noncarrier [C57BL/6NJcl]
ReferencesCell, 132, 487-498 (2008). 18267078
Proc. Natl. Acad. Sci. USA, 107(27)12192-12197(2010). 20547847

Health Report

Examination Date / Room / Rack2016/04/18Room:3-CRack:E
2016/02/15Room:3-CRack:E
2015/12/14Room:3-CRack:E
2015/10/19Room:3-CRack:E
2015/08/17Room:3-CRack:E
2015/06/15Room:3-CRack:E
2015/04/20Room:3-CRack:E
2015/02/16Room:3-CRack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GMNNgeminin (human)UNGMNN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
mAGmonomeric Azami Green (Coral)UNmAGCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Coral mAG(monomeric Azami Green), human Geminin
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement regarding the usage of the CAG promoter specified by the DEVELOPER, Dr. Junichi Miyazaki <FAX: +81-6-6879-3829, e-mail: jimiyaza@nutri.med.osaka-u.ac.jp> of Osaka University. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a non-for profit institution for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Proc. Natl. Acad. Sci. U S A., 107 (27): 12192-12197 (2010) designated by the DEPOSITOR is requested. The RECIPIENT agrees to acknowledge PROVIDER in all oral presentations and written publications as related to the MATERIAL and provide a copy of the publication to the DEPOSITOR.
DepositorAtsushi Miyawaki (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
CAGGS MATERIAL TRANSFER AGREEMENT (Pdf)/ (Word)
Miyawaki Lab HP
Genetic Background

BRC mice in Publications

Marchingo JM, Kan A, Sutherland RM, Duffy KR, Wellard CJ, Belz GT, Lew AM, Dowling MR, Heinzel S, Hodgkin PD.
T cell signaling. Antigen affinity, costimulation, and cytokine inputs sum linearly to amplify T cell expansion.
Science 346(6213) 1123-7(2014) 25430770