|Former Common name||B6.Cg-Tg(Fucci)474Bsi, B6;B6D2-Tg(Fucci)474Bsi, B6;B6D2-Tg(Fucci)474Bsi|
|ES Cell line|
|Appearance||black [a/a B/B C/C]|
|Strain development||Developed by Asako Sakaue-Sawano and Atsushi Miyawaki, RIKEN Brain Science Institute. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6N.|
|Strain description||Transgenic mouse strains express fucci (fluorescent, ubiquitination-based cell cycle indicator) under the control of CAG promoter. These mice can be used to clearly visualize the cell cycle with fluorescence, i.e. S/G2/M phase nuclei green and those in G1 phase red in vivo. #474 (RBRC02704), #492 (RBRC02705), #504 (RBRC02706): Fucci-S/G2/M-Green (mAG-hGeminin(1/110)). #596 (RBRC02707), #610 (RBRC02708), #639 (RBRC02709): Fucci-G1-Red (mKO2-hCdt1(30/120)). For whole body (various tissues): #504 and #596 (Cell 2008). For hematopoietic system (monocyte and macrophage): #474 and #610 (Development 2013). For lymphocytic system: #492 and 639 (PNAS 2010). #474 and #610 double transgenic mice (RBRC02817). #504 and #596 double transgenic mice (RBRC02892). Whole-body visualization of Fucci mice with a flashlight (PCR genotyping is also available.): Check the Fucci S/2G/M-Green expressing mice at 1-4 days after birth by using blue LED flashlight through a sharp-cut filter in the dark box. Check the Fucci G1-Red expressing mice within 1-4 days after birth by using green LED flashlight through a green band-cut filter in the dark box. The part with few hairs such as in the mouth and auricles is suitable for the observation of fluorescence. Visualizing device: blue LED flashlight (470 nm), S470/30 filter, 535AF26 filter, sharp-cut filter (500 or 520 nm), green band-cut filter (red filter). #474 and #504: Tg homozygous mice are viable and fertile. #596: Tg homozygous mice are probably lethal (details unknown). #492, #610 and #639: Viability and fertility of homozygous mice are unknown (probably decreased fertility, details unknown).|
|Colony maintenance||Carrier x Carrier [or Crossing to C57BL/6NJcl]|
|References||Development, 140(22):4624-32 (2013) doi:10.1242/dev.099226 24154524|
Cell, 132, 487-498 (2008). 18267078
PLOS ONE, 8(9):e73801 (2013). 24066072
|Examination Date / Room / Rack||2020/08/24Room:3-CRack:A|
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gene symbolGene nameChr.Allele symbolAllele namePromoter
mAGmonomeric Azami Green (Coral)UNmAGCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
|Donor DNA||CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Coral mAG(monomeric Azami Green) cDNA, human Geminin cDNA|
|Research application||Fluorescent Proteins/lacZ System|
|Specific Term and Conditions||In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLOS ONE (2013) doi: 10.1371/journal.pone.0073801. Development (2013) doi:10.1242/dev.099226.The RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT agrees to acknowledge PROVIDER in all oral presentations and written publications as related to the MATERIAL and provide a copy of the publication to the DEPOSITOR. The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement regarding the usage of the CAG promoter specified by the DEVELOPER, Dr. Junichi Miyazaki <FAX: +81-6-6879-3829, e-mail: email@example.com> of Osaka University.|
|Depositor||Atsushi Miyawaki (RIKEN)|
|Strain Status||Live mice|
|Strain Availability||Cryopreserved sperm (within 1 month)|
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
|Additional Info.||Genotyping protocol -PCR-|
CAGGS MATERIAL TRANSFER AGREEMENT (Pdf)/ (Word)
Miyawaki Lab HP
BRC mice in Publications
Yo M, Sakaue-Sawano A, Noda S, Miyawaki A, Miyoshi H.
Fucci-guided purification of hematopoietic stem cells with high repopulating activity.
Biochem. Biophys. Res. Commun. (2014) 25534850
Morgane Sonia Thion, Donovan Low, Aymeric Silvin, Jinmiao Chen, Pauline Grisel, Jonas Schulte-Schrepping, Ronnie Blecher, Thomas Ulas, Paola Squarzoni, Guillaume Hoeffel, Fanny Coulpier, Eleni Siopi, Friederike Sophie David, Claus Scholz, Foo Shihui, Josephine Lum, Arlaine Anne Amoyo, Anis Larbi, Michael Poidinger, Anne Buttgereit, Pierre-Marie Lledo, Melanie Greter, Jerry Kok Yen Chan, Ido Amit, Marc Beyer, Joachim Ludwig Schultze, Andreas Schlitzer, Sven Pettersson, Florent Ginhoux, Sonia Garel.
Microbiome Influences Prenatal and Adult Microglia in a Sex-Specific Manner.
Cell 172(3) 500?516(2018) 29275859
Evrard M, Kwok IWH, Chong SZ, Teng KWW, Becht E, Chen J, Sieow JL, Penny HL, Ching GC, Devi S, Adrover JM, Li JLY, Liong KH, Tan L, Poon Z, Foo S, Chua JW, Su IH, Balabanian K, Bachelerie F, Biswas SK, Larbi A, Hwang WYK, Madan V, Koeffler HP, Wong SC, Newell EW, Hidalgo A, Ginhoux F, Ng LG.
Developmental Analysis of Bone Marrow Neutrophils Reveals Populations Specialized in Expansion, Trafficking, and Effector Functions.
Immunity (2018) 29466759
Takegahara N, Kim H, Mizuno H, Sakaue-Sawano A, Miyawaki A, Tomura M, Kanagawa O, Ishii M, Choi Y.
Involvement of receptor activator of nuclear factor kappa-B ligand-induced incomplete cytokinesis in polyploidization of osteoclasts.
J. Biol. Chem. (2015) 26670608
Shand FH, Ueha S, Otsuji M, Koid SS, Shichino S, Tsukui T, Kosugi-Kanaya M, Abe J, Tomura M, Ziogas J, Matsushima K.
Tracking of intertissue migration reveals the origins of tumor-infiltrating monocytes.
Proc. Natl. Acad. Sci. U.S.A. (2014) 24825888