RBRC02697

Information1

Image
BRC No.RBRC02697
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Rapgef2<tm1Tkat>
Former Common nameRA-GEF-1<flox/flox>
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Tohru Kataoka, Kobe University Graduate School of Medicine in 2007. E14TG2a ES cells were used to generate the mutant mice. The mice were crossed to C57BL/6 over 8 times.
Strain descriptionRA-GEF-1 (Rapgef2) gene floxed mice. Exon 15 was flanked by loxP sites. Homozygous mice are viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesBiochem. Biophys. Res. Commun., 363, 106-112 (2007). 17826737

Health Report

Examination Date / Room / Rack2009/11/16Room:4-ARack:E
2009/09/14Room:4-ARack:E
2009/07/13Room:4-ARack:E
2009/05/19Room:4-ARack:E
2009/05/18Room:4-ARack:E
2009/03/16Room:4-ARack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Rapgef2Rap guanine nucleotide exchange factor (GEF) 23Rapgef2<tm1Tkat>targeted mutation 1, Tohru Kataoka

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP3loxP

Information2

Donor DNAPhage P1 loxP sites, mouse Rapgef2 genomic DNA
Research applicationCell Biology Research
Cre/loxP system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 363, 106-112 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorTohru Kataoka (Kobe University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data