Strain Data Sheet

RBRC02692

Information1

Image
BRC No.RBRC02692
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129X1-Lilrb4a<tm1Ttk>
Former Common namegp49B
H-2 Haplotype
ES Cell lineRW4 [129X1/SvJ]
Background strainC57BL/6NCrlCrlj
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Toshiyuki Takai, Tohoku University in 2008.
Strain descriptionLilrb4 knock-out mice. Lilrb4 (gp49B) is a member of the immunoglobulin superfamily, containing immunoreceptor tyrosine-based inhibitory motifs that can deliver an inhibitor signal in mast and NK cells.
Colony maintenanceSibling x Sibling (Homozygote x Homozygote)Homozygous mice are viable and fertile.
ReferencesJ. Biol. Chem., 273, 1070-1074 (1998). 9422771
Eur. J. Immunol., 38 (9) 2426-37 (2008). 18792399
J. Immunol., 165, 4970-4977 (2000). 11046024

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Lilrb4aleukocyte immunoglobulin-like receport, subfamily B, member 410Lilrb4a<tm1Ttk>targeted mutation 1, Toshiyuki TakaiCD85K, Gp49b, HM18, ILT3, Lilrb4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)10neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Lilrb4a genomic DNA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 273, 1070-1074 (1998). J. Immunol., 165, 4970-4977 (2000).
DepositorToshiyuki Takai (Tohoku University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Li Z, Zhao M, Li T, Zheng J, Liu X, Jiang Y, Zhang H, Hu X.
Decidual Macrophage Functional Polarization during Abnormal Pregnancy due to Toxoplasma gondii: Role for LILRB4.
Front Immunol 8 1013(2017) 28883820