Strain Data Sheet


Strain Information

BRC No.RBRC02655
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;129-Xist<tm5Sado>
Former Common nameXist<delta A>; B6.Cg-Xist<tm5Sado>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Strain developmentDeveloped by Takashi Sado, National Institute of Genetics in 2007. R1 ES cells were used to generate the mutant mice. A loxP flanked neo cassette was removed by crossing with cre mice (B6).
Strain descriptionXist<delta> mice. The portion of exon 1 encoding the A repeat was deleted. Heterozygous mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655).
Colony maintenanceHeterozygote (female) x Wild-type (male) [C57BL/6JJcl]
ReferencesDevelopment, 136 (1) 139-146 (2009). 19036803

Health Report

Examination Date / Room / Rack2024/05/20Room:4-ARack:D


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Xistinactive X specific transcriptsXXist<tm5Sado>targeted mutation 5, Takashi Sado

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Ordering Information

Donor DNAPhage P1 loxP site, mouse Xist genomic DNA
Research applicationCre/loxP system
Developmental Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorTakashi Sado (National Institute of Genetics)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
Strain AvailabilityCryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-
Mouse of the Month Feb 2009

BRC mice in Publications

Fukuda A, Mitani A, Miyashita T, Sado T, Umezawa A, Akutsu H.
Maintenance of Xist Imprinting Depends on Chromatin Condensation State and Rnf12 Dosage in Mice.
PLoS Genet 12(10) e1006375(2016) 27788132