RBRC02599

Information1

Image
BRC No.RBRC02599
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameIVCS.129P2(B6)-Akr1c18<tm1Mni>
Former Common name20a-HSD IVCS
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainIVCS
Appearance
Strain development
Strain description
Colony maintenance
ReferencesJ. Reprod. Dev., 53, 499-508 (2007) 17272929

Health Report

Examination Date / Room / Rack2020/02/03Room:3-2Rack:A
2019/12/02Room:3-2Rack:A
2019/10/16Room:3-2Rack:A
2019/08/05Room:3-2Rack:A
2019/06/03Room:3-2Rack:A
2019/04/01Room:3-2Rack:A
2019/02/04Room:3-2Rack:A
2018/12/03Room:3-2Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Akr1c18aldo-keto reductase family 1, member C1813Akr1c18<tm1Mni>targeted mutation 1, Masugi Nishihara

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)13

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)13

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Jellyfish EGFP cDNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, bovine Growth Hormone poly-A signal,mouse 20 alpha-hydroxysteroid dehydrogenase genomic DNA
Research applicationFluorescent Proteins/lacZ System
Reproductive Biology Research
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorMasugi Nishihara (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data