Strain Data Sheet

RBRC02573

Strain Information

Image
BRC No.RBRC02573
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Traf5<tm1Hna>
Former Common nameB6.traf5<-/->, TRAF5 KO,C57BL/6
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Hiroshi Nakano and Haruhio Koseki in 1999.
Strain descriptionHomozygous mutant mice show defects in lymphocyte activation.
Colony maintenanceSibling Mating (Homozygote x Homozygote)Homozygous mutant mice are viable and fertile.
References
Targeted disruption of Traf5 gene causes defects in CD40- and CD27-mediated lymphocyte activation.
Nakano H, Sakon S, Koseki H, Takemori T, Tada K, Matsumoto M, Munechika E, Sakai T, Shirasawa T, Akiba H, Kobata T, Santee S M, Ware C F, Rennert P D, Taniguchi M, Yagita H, Okumura K
Proc. Natl. Acad. Sci. USA, 96, 9803-9808 (1999). 10449775

Critical roles of TRAF2 and TRAF5 in tumor necrosis factor-induced NF-kappa B activation and protection from cell death.
Tada K, Okazaki T, Sakon S, Kobarai T, Kurosawa K, Yamaoka S, Hashimoto H, Mak T W, Yagita H, Okumura K, Yeh W C, Nakano H
J. Biol. Chem., 276, 36530 (2001) 11479302

TNF receptor-associated factor 5 limits the induction of Th2 immune responses.
So T, Salek-Ardakani S, Nakano H, Ware C F, Croft M
J. Immunol., 172, 4292-4297 (2004). 15034043

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Traf5TNF receptor-associated factor 51Traf5targeted mutation 1, Hiroyasu Nakano
neoneomycin resistance gene (E. coli)1herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal B cell proliferation(MP:0005153)

  • abnormal T cell proliferation(MP:0005094)

  • abnormal humoral immune response(MP:0001800)

  • decreased IgG1 level(MP:0008495)

  • decreased IgM level(MP:0001806)
  • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli neo, mouse traf5 genomic DNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter)
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The recipient of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
    DepositorHiroyasu Nakano (Juntendo University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Wang L, Lu Y, Guan H, Jiang D, Guan Y, Zhang X, Nakano H, Zhou Y, Zhang Y, Yang L, Li H.
    Tumor necrosis factor receptor-associated factor 5 is an essential mediator of ischemic brain infarction.
    J Neurochem 126(3) 400-14(2013) 23413803