RBRC02566

Information1

Image
BRC No.RBRC02566
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Nanos3<tm3(lacZ)Ysa>/YsaRbrc
Former Common nameNanos3-LpA(neo)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesDev Biol., 336(2):222-31 (2009). 19818747
Saga Y., 2008. Mouse nanos proteins are essential for germ cell development. In Genetics and Epigenetic Control of Mammalian Germ Cell Development and Function (Nozaki M. edited.) Research Singpost, Kerala, India, 2008.

Health Report

Examination Date / Room / Rack2009/04/07Room:3-7Rack:B
2009/04/06Room:3-7Rack:B
2009/02/09Room:3-7Rack:B
2008/12/08Room:3-7Rack:B
2008/10/06Room:3-7Rack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Nanos3nanos homolog 3 (Drosophila)8Nanos3<tm3(lacZ)Ysa>targeted mutation 3 (lacZ), Yumiko Saga

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)8

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)8mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAE. coli lacZ, SV40 pA, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Nanos3 genomic DNA
Research applicationCre/loxP system
Developmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Saga Y., 2008. Mouse nanos proteins are essential for germ cell development. In Genetics and Epigenetic Control of Mammalian Germ Cell Development and Function (Nozaki M. edited.) Research Singpost, Kerala, India, 2008.Within a period of two years after deposition of the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and/or the DEVELOPER's names as co-authors.
DepositorYumiko Saga (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Saga Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data