RBRC02565

Information1

Image
BRC No.RBRC02565
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Nanos3<tm2(cre)Ysa>/35YsaRbrc
Former Common nameNanos3-CrepA(neo)#35
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Yumiko Saga (2007).
Strain descriptionThese knock-in mice have a cre gene at the Nanos3 locus. Expression is detected in the early PGC and testis. Homozygous mutant mice are infertile.
Colony maintenanceSibling Mating (Hemizygote x Wild-type)
ReferencesDev. Biol., 318, 133-142 (2008). 18436203

Health Report

Examination Date / Room / Rack2009/06/22Room:3-BRack:L
2009/04/21Room:3-BRack:L
2009/04/20Room:3-BRack:L
2009/02/23Room:3-BRack:L
2008/12/15Room:3-BRack:L
2008/10/20Room:3-BRack:L
2008/08/25Room:3-BRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Nanos3nanos homolog 3 (Drosophila)8Nanos3<tm2(cre)Ysa>targeted mutation 2 (cre), Yumiko Saga

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinase8cre

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)8mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 cre recombinase, SV40 poly A, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, yeast FRT (flipase recombination target) site, mouse nanos3 genomic DNA
Research applicationCre/loxP system
Developmental Biology Research
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 318, 133-142 (2008).Within a period of two years after deposition of the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and/or the DEVELOPER's names as co-authors.
DepositorYumiko Saga (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Saga Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data