RBRC02498

Information1

Image
BRC No.RBRC02498
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Tg(Stra8-EGFP)1Ysa/YsaRbrc
Former Common nameBAC-stra8EGFP-bghpA#1
H-2 Haplotype
ES Cell line
Background strain
AppearanceUnfixed
Strain developmentDeveloped by Dr. Yumiko Saga, National Institute of Genetics in 2007. ICR mixed background.
Strain descriptionBAC transgenic mice having a fusion gene of Stra8 and EGFP, which also contain a beta-globin polyA signal.
Colony maintenanceCarrier x NoncarrierTg mice are viable and fertile.According to a breeding result, the transgene may be integrated into chromosome Y.
ReferencesBiol Reprod., 89(1):3 (2013). 23677978

Health Report

Examination Date / Room / Rack2010/05/10Room:2-3Rack:C
2008/12/15Room:3-CRack:G
2008/10/20Room:3-CRack:G
2008/08/25Room:3-CRack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Stra8stimulated by retinoic acid gene 8UNStra8

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBB2beta-globin polyA signalUNHBB2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)UN

Information2

Donor DNAJellyfish EGFP cDNA, beta-globin-polyA signal, mouse Stra8 genomic DNA, yeast FRT (flipase recombination target) sites, E. coli neo, DNA from BAC clone RP23-367A8
Research applicationDevelopmental Biology Research
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. Within a period of two years after deposition of the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and/or the DEVELOPER's names as co-authors.
DepositorYumiko Saga (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Saga Lab HP

BRC mice in Publications

Chihara M, Ikebuchi R, Otsuka S, Ichii O, Hashimoto Y, Suzuki A, Saga Y, Kon Y.
Mice stage-specific claudin 3 expression regulates progression of meiosis in early stage spermatocytes.
Biol. Reprod. 89(1) 3(2013) 23677978