RBRC02492

Information1

Image
BRC No.RBRC02492
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Mesp1/Mesp2<tm1Ysa>Tg(deltaP2deltaEME)2Ysa/YsaRbrc
Former Common nameBAC- Delta P2 Delta EME#2/Mesp1 Mesp2-dko
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesMech. Dev., 125, 432-440 (2008) 18328678

Health Report

Examination Date / Room / Rack2009/07/06Room:4-2Rack:C
2009/05/12Room:4-2Rack:C
2009/05/11Room:4-2Rack:C
2009/03/09Room:4-2Rack:C
2009/01/13Room:4-2Rack:C
2008/11/10Room:4-2Rack:C
2008/09/08Room:4-2Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Mesp1/Mesp2mesoderm posterior 17Mesp1/Mesp2<tm1Ysa>targeted mutation 1, Yumiko Saga

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
ALPalkaline phosphatase (human)UNALP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Information2

Donor DNAhuman alkaline phosphatase-pA cDNA, yeast Frt, BAC(Bacterial artificial chromosome)clone containing mouse Mesp1 genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo
Research applicationDevelopmental Biology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mech Dev., 125, 432-440 (2008).
DepositorYumiko Saga (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Saga Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data