Strain Data Sheet

RBRC02467

Information1

Image
BRC No.RBRC02467
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Hprt1<tm1Mok>
Former Common nameHPRT-KO, B6-Hprt KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Motoya Katsuki, National Institutes of Natural Sciences.
Strain descriptionHprt gene knockout mice.
Colony maintenanceHeterozygote (female), Hemizygote (male) x Wild-type [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hprt1hypoxanthine guanine phosphoribosyl transferaseXHprt1<tm1Mok>targeted mutation 1, Motoya Katsuki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)Xherpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Hprt genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 未定In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorToshikuni Sasaoka (Niigata University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

Aizawa S, Nishimura K, Mondejar GS, Kumar A, Bui PL, Tran YTH, Kuno A, Muratani M, Kobayashi S, Nabekura T, Shibuya A, Sugihara E, Sato TA, Fukuda A, Hayashi Y, Hisatake K.
Early reactivation of clustered genes on the inactive X chromosome during somatic cell reprogramming.
Stem Cell Reports 17(1) 53-67(2022) 34919813