RBRC02443

Information1

Image
BRC No.RBRC02443
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Akr1c18<tm1Mni>
Former Common name20alpha-HSD knock-out mouse
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesJ. Reprod. Dev., 53, 499-508 (2007) 17272929

Health Report

Examination Date / Room / Rack2009/06/22Room:3-DRack:A
2009/04/21Room:3-DRack:A
2009/04/20Room:3-DRack:A
2009/02/23Room:3-DRack:A
2008/12/15Room:3-DRack:A
2008/10/20Room:3-DRack:A
2008/08/25Room:3-DRack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Akr1c18aldo-keto reductase family 1, member C1813Akr1c18<tm1Mni>targeted mutation 1, Masugi Nishihara

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)13

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)13

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAマウス20α-水酸化ステロイド脱水素酵素遺伝子ゲノム断片、IRES、EGFP、ネオマイシン耐性遺伝子
Research applicationReproductive Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Reprod. Dev., 53, 499-508 (2007).The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorMasugi Nishihara (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data