RBRC02435

Information1

Image
BRC No.RBRC02435
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Srpx<tm1Hzi>
Former Common nameDrs KO, Srpx KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Yukihiro Tambe and Hirokazu Inoue, Shiga University of Medical Sciences in 2007.
Strain descriptionThe Drs gene is originally found as a novel suppressor of v-src transformation. The Drs gene expresses in most normal tissues, however the expression is completely downregulated in a variety of cancer cell lines and tissues. It has been shown that malignant tumors such as lymphomas, lung adenocarcinomas and hepatomas were generated in about 30% of the Drs KO mice between 7 and 12 months. The Drs KO embryonic fibroblasts also showed enhanced sensitivity to transformation by viral oncogenes. Reintroduction of Drs gene into a tumor cell line derived from the tumor of the Drs KO mouse suppresses tumorigenesis and enhances caspase-mediated apoptosis.
Colony maintenanceHeterozygote (female), Hemizygote (male) x C57BL/6 [C57BL/6JJcl]
ReferencesCarcinogenesis, 28, 777-784 (2007). 17088259

Health Report

Examination Date / Room / Rack2011/12/05Room:3-8Rack:A
2011/10/03Room:3-8Rack:A
2011/08/08Room:3-8Rack:A
2011/07/04Room:4-5Rack:A
2011/04/25Room:4-5Rack:A
2011/03/07Room:4-5Rack:A
2011/03/07Room:4-5Rack:A
2011/01/11Room:4-5Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Srpxsushi-repeat-containing proteinXSrpx<tm1Hzi>targeted mutation 1, Hirokazu Inoue

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPXloxP

Information2

Donor DNAmouse srpx genomic DNA, Phage P1 loxP
Research applicationApoptosis Research
Cancer Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Carcinogenesis, 28(4), 777-784 (2007).The RECIPENT uses the BIOLOGICAL RESOURCE as a collaborative project with the DEPOSITOR.
DepositorHirokazu Inoue (Shiga University of Medical Science)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-
Mouse of the Month Jun 2008

BRC mice in Publications

No Data