RBRC02421

Information1

Image
BRC No.RBRC02421
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Bcl11b<tm1Jpk>
Former Common nameBcl11b/Rit1-KO mice
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearancealbino [A/A b/b c/c]
Strain developmentDeveloped by Ryo Kominami, Graduate School of Medical and Dental Science, Niigata University. TT2 ES cells derived from B6CBAF1 were used for the generation of knockout mice. C57BL/6 background (RBRC06231), BALB/c background (RBRC02421).
Strain descriptionBcl11b gene knock-out mice. A PGK-neo cassette was inserted into exon 1 of the Bcl11b gene. Bcl11b is a tumor-suppressor gene involved in T cell lymphomas. Homozygous mutant mice diw within the first day after birth. Mutant mice show a block at the CD4-CD8 double-negative stage of thymocyte development without any impairment in cells of B- or gammadelta T cell lineages. C57BL/6 background (RBRC06231), BALB/c background (RBRC02421).
Colony maintenanceHeterozygote x Wild-type [BALB/cAJcl]
ReferencesNat. Immunol., 4, 533-539 (2003) 12717433

Health Report

Examination Date / Room / Rack2013/03/25Room:4-BRack:B
2013/01/21Room:4-BRack:B
2012/11/26Room:4-BRack:B
2012/11/21Room:4-BRack:B
2012/11/19Room:4-BRack:B
2012/09/24Room:4-BRack:B
2012/07/23Room:4-BRack:B
2012/07/04Room:4-BRack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Bcl11bB-cell leukemia/lymphoma 11B12Bcl11b<tm1Jpk>targeted mutation 1, Japan Kominami

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)12mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse Bcl11b genome DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter)
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Immunol., 4, 533-539 (2003).The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorRyo Kominami (Niigata University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data