RBRC02415

Information1

Image
BRC No.RBRC02415
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(TRE-GFP)392Yasu
Former Common nameβ-catenin/Tcf reporter mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Yasuhiro Yamada, Gifu University Graduate School of Medicine in 2007. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. Line 392 (main line) (RBRC02415), line 622 (rbrc02416) and line 628 (RBRC02417).
Strain descriptionBeta-catenin/Tcf reporter mice. These mice express GFP gene under the control of the Tcf/Lef binding sites and a minimal thymidine kinase promoter from the TOPFLASH plasmid. Immunohistochemistry with a GFP antibody can be used to assess the Tg expression in the intestine. In other organs except for the intestine. In the splenocytes, expression of GFP was observed by FACS analysis, In other organs, the GFP expression patterns are not examined yet.
Colony maintenance
ReferencesCancer Res., 67, 4079-4087 (2007). 17483318
Carcinogenesis, 29, 666-672 (2008). 18204079

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNTcf binding element, herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signalUN

Information2

Donor DNATcf/Lef binding site (TOPFLASH plasmid, upstate社, 人工的?), TK promoter (HSV), EGFP (jellyfish), intron (SV40), polyA (SV40)
Research applicationCancer Research
Cell Biology Research
Developmental Biology Research
Fluorescent Proteins/lacZ System
Tet system
General Purpose
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
DepositorYasuhiro Yamada (Gifu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data