RBRC02412

Information1

Image
BRC No.RBRC02412
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Nes-cre)1Nogu
Former Common nameB6.Cg-Tg(Nes-cre)1Nogu
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Shigeru Noguchi, Meiji Dairies Corporation and Kanagawa Academy of Science and Technology and Dr. Toshikuni Sasaoka, Center for Bioresource-based Researches, Brain Research Institute, Niigata University. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6.
Strain descriptionNestin-cre transgenic mice, expressing a cre recombinase gene under the control of Nestin promoter and enhancer. This Nestin-cre transgene contains a rat nestin promoter, cre recombinase gene, IRES-lacZ-polyA and neuron specific enhancer (around 100 bp of exon 1, the entire intron 1 and around 100 bp of exon 3 of nesitin gene). Tissue-specific expression: observed in whole brain, spinal cord and testis/ovary; not observed in thymus, lung, heart, kidney, spleen, stomach, small and large intestine, pancreas, skeletal muscle and tail (Developers, personal communication).
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6JJcl)
References

Health Report

Examination Date / Room / Rack2020/03/30Room:4-ARack:C
2020/01/27Room:4-ARack:C
2019/11/25Room:4-ARack:C
2019/09/30Room:4-ARack:C
2019/07/29Room:4-ARack:C
2019/05/27Room:4-ARack:C
2019/03/25Room:4-ARack:C
2019/01/28Room:4-ARack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
NesnestinUNNes

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinaseUNcre

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
poly ASV40 polyA signalUNpoly A

Information2

Donor DNARat nestin genomic DNA, phage P1 cre recombinase, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires) sequence, Simian virus 40 Large T antigen nuclear localization signal (NLS), E. coli lacZ, SV40 poly A
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature(s) designated by the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of academic research. Prior to publication, the RECIPIENT should contact the developer scientist, Dr. Shigeru Noguchi and acknowledge Drs. Shigeru Noguchi and Dr. Toshikuni Sasaoka.
DepositorKoichiro Nishimura (Kanagawa Academy of Science and Technology)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Trifonov S, Yamashita Y, Kase M, Maruyama M, Sugimoto T.
Overview and assessment of the histochemical methods and reagents for the detection of -galactosidase activity in transgenic animals.
Anat Sci Int (2015) 26394634

Tanaka K, Furuyashiki T, Kitaoka S, Senzai Y, Imoto Y, Segi-Nishida E, Deguchi Y, Breyer RM, Breyer MD, Narumiya S.
Prostaglandin e2-mediated attenuation of mesocortical dopaminergic pathway is critical for susceptibility to repeated social defeat stress in mice.
J. Neurosci. 32(12) 4319-29(2012) 22442093