Strain Data Sheet

RBRC02410

Strain Information

Image
BRC No.RBRC02410
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6Cr-Tg(Pcdh21-creER)CYoko
Former Common namePcdh21-CreER
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Mineto Yokoi, Kyoto University Graduate School of Medicine in 2005.
Strain descriptionC57BL/6Cr-Tg(Pcdh21-creER)CYoko. This transgenic mice express the tamoxifen-inducible cre recombinase gene under the control of the mouse protocadherin 21 gene promoter. This can be used to make an conditional knock-out by crossing with the other genetically-engineered mice that have floxed genes. The expression of cre recombinase was observed in mitral/tufted cells.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6CrSlc)
References
Conditional genetic labeling of mitral cells of the mouse accessory olfactory bulb to visualize the organization of their apical dendritic tufts.
Yonekura J, Yokoi M
Mol. Cell. Neurosci., 37, 708-718 (2008). 18201899

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Esr1* estrogen receptor 1 (ER)UNEsr1*
HBB2 rabbit beta-globin intron/polyA signalUNHBB2
cre Phage P1 Cre recombinaseUNcre mouse Pcdh21 promoter

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • no abnormal phenotype detected (MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse pcdh21 promoter, phage P1 Cre, mouse estrogen receptor cDNA, rabbit beta-globin intron/polyA signal
    Research applicationCre/loxP system
    Neurobiology Research
    Cre driver
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Neurosci., 37(4):708-718 (2008). RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
    DepositorYokoi Mineto (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data