RBRC02405

Information1

Image
BRC No.RBRC02405
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-4930583H14Rik<tm1Hmd>
Former Common name4930583H14Rik flox
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Takako Maeda and Hiroshi Hamada, Institute for Molecular and Cellular Biology, Osaka University in 2007. G4 ES cells derived from (129S6 x C57BL/6)F1 were used. C57BL/6 and 129 mixed background.
Strain description4930583H14Rik floxed mice. ORF region of the exon 4 was flanked by loxP sites.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2008/09/08Room:4-5Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
4930583H14RikRIKEN cDNA 4930583H14 gene34930583H14Rik<tm1Hmd>targeted mutation 1, Hiroshi Hamada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)3

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)3

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
myc-tagmyc-epitope tag3myc-tag

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)3mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, epitope tag(mycTAG), yeast FRT (flipase recombination target) site, Phage P1 Cre recombination target sites(loxp), E. coli neo
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorHiroshi Hamada (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data